Abstract
Eight species of the genus Symplocos have been recorded in Colombia including Symplocos theiformis, called by Mutis “té de Bogotá” due to its astringent and tonic qualities similar to tea and coffee. It has a high potential for use and has a restricted distribution in the rural areas of the Capital District, for which its propagation and conservation are very important. The objective of the present study was to evaluate the tolerance to desiccation and the existence of physiological dormancy in the seeds. For this, under controlled greenhouse conditions, the germination percentage and the average germination time were evaluated in scarified and fresh seeds with a humidity content of 10% and 5% and with gibberellic acid (AG3: 400 ppm). As a result, with all treatments, germination started in 97 days; the germination percentage for fresh seeds with 10% and 5% humidity contents and AG3 was 41.7%, 26.7%, 28.3%, and 18.3%; the average germination time was 213.8, 210.6, 194.1, and 211.5 days, respectively. Their daily growth rate was 0.12 mm/day, while the seedlings were 4.2 cm high after 183 days and 92.1% had survived after 363 days. There were no significant statistical differences between treatments in the germination percentage (p=0.2309) nor in the average germination time (p=0.4517). Although there was no difference between treatments, given the decrease in the germination power of the seeds, it can be said that they are recalcitrant to storage, their germination is slow and asynchronous over time, their sexual propagation is viable, and their long-term ex situ conservation is feasible in living collections, seedling banks or in vitro germplasm banks.
References
Badoni, R., Senwal, K., Kothiyal, K., Rawat, U. (2010). Chemical constituents and biological applications of the genus Symplocos. Journal of Asian natural products research. 12: 1069-1080.
Bernal, H.Y., García, M.H., Quevedo, S.F. (2011). Pautas para el conocimiento, conservación y uso sostenible de las plantas medicinales nativas en Colombia: estrategia nacional para la conservación de plantas. Ministerio de Ambiente, Vivienda y Desarrollo Territorial e Instituto de Investigación de Recursos Biológicos Alexander von Humboldt. Bogotá, D. C., Colombia. 232 p.
Bewley, J.D. & Black, M. (1994). Seeds: Physiology of development and germination. Plenum Press, New York. 445 p. Bewley, J. D., Bradford, K. J., Hilhorst, W. M., Nonogaky, H. (2013). Seed. Physiology of development, germination and dormancy. Third Edition. Springer. New York, U.S.A. 392 p.
Bradford, K.J. & Nonogaki, H. (2007). Seed development, dormancy and germination. First edition. Oxford, Blackwell Publishing. p. 224-247.
Cabezas, L. (2016). Protocolo de propagación sexual y asexual de Symplocos theiformis (L. f.) Oken en la ciudad de Bogotá D.C. (Tesis de pregrado). Facultad de Ciencias Agrarias, Universidad Nacional de Colombia sede Bogotá, Bogotá, Colombia. 49 p.
Carvalho, N. & Nakagawa, J. (2000). Sementes: ciencia, tecnología e producao. 4. ed. Joboticabal: Funep, 588p. Carvalho, P. (2008). Maria-Mode-do-Banhado (Symplocos uniflora). Embrapa Circular Técnica (148) 6p. ISSN 1517-5278.
Corbineau, F., Xia, Q., Bailly C., El-Maarouf-Bouteau, H. (2014). Ethylene, a key factor in the regulation of seed dormancy. Front. Plant Science. 5: 1-13. Doi: 10.3389/fpls.2014.00539
Córdoba, S. (2007). Compilación y análisis de la información relacionada con la propagación de especies enmarcadas en el proyecto de uso sostenible de los recursos vegetales del Distrito Capital y la Región. Informe técnico inédito. Bogotá, D. C.: Jardín Botánico José Celestino Mutis-Subdirección Científica. 106 p.
Córdoba, S., Guzmán-C, J., Pérez-M, B., Zúñiga-Upegui, P., Pacheco, R. (2010). Propagación de especies nativas de la región andina. Subdirección Científica. Jardín Botánico José Celestino Mutis. Bogotá D.C. p. 209-212.
Di Renzo, J.A., Casanoves, F., Balzarini, M.G., González, L., Tablada, M., Robledo, C.W. InfoStat (versión 2008), Grupo InfoStat, FCA, Universidad Nacional de Córdoba, Argentina. p. 167-231.
Doria, J. (2010). Generalidades sobre las semillas: su reproducción, conservación y almacenamiento. Cultivos Tropicales. 31 (1): 00. Fecha de consulta: 6 de mayo de 2019. Disponible en: http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S0258-59362010000100011&lng=es&tlng=es
Hastwell G.T. & Facelli J.M. (2003). Differing effects of shade induced facilitation on growth and survival during the establishment of a chenopod shrub. Journal of Ecology. 91:941-950. Doi: 10.1046/j.1365-2745.2003.00832.x
Hong D.T. & Ellis R.H. (2002). Storage. Chapter 3. In: Vozzo, J.A. (Ed.) 2002, Tropical tree seed manual. Washington DC, USDA Forest Service. International Seed Testing Association - ISTA (2017). International Rules for Seed Testing. The International Seed Testing Association, Bassersdorf, Switzerland. Fecha de consulta: 3 de abril de 2018. Disponible en: https://www. seedtest.org/en/ista-rules-for-2017-_content---1--1448.html
Farooq, U., Khan, S., Naz, S. Khan, A., Khan A., Ahmed A., Rauf, A., Bukhari S.M., Khan S.A., Kamil A., Riaz, N., Kahan A.R. (2017). Three new anthraquinone derivatives isolated from Symplocos racemosa and their antibiofilm activity. Chinese Journal of Natural Medicines. 15 (12): 0944-0949.
Kar, D. & Kumar, M. (2017). In vitro antioxidant potential of methanolic extract of Symplocos racemosa Roxb. Asian Journal of Chemistry. 29 (10): 2271-2274.
Kelly, L.M. (2018). Symplocos theiformis (L.f.) Oken. En Bernal, R., S.R. Gradstein & M. Celis (editores). 2015. Catálogo de plantas y líquenes de Colombia. Instituto de Ciencias Naturales, Universidad Nacional de Colombia, Bogotá. Fecha de consulta: 28 de junio de 2018. Disponible en: http://catalogoplantasdecolombia.unal.edu.co
Linero, J. C. (2006). Desarrollar los protocolos de propagación in vitro de tres especies andinas priorizadas y complementar, validar y propagar el protocolo de propagación de la especie Symplocos theiformis, que se viene trabajando en el marco del proyecto de uso sostenible de los recursos vegetales del Distrito Capital y la Región. Informe técnico inédito. Bogotá, D.C.: Jardín Botánico de Bogotá José Celestino Mutis–Subdirección Científica. 97 p.
Liu, Q., Sun, Y., Chen, J., Li, P., Li, C., Jiang, L., Niu, G. (2017). Symplocos paniculata: A new ornamental plant species. Acta Hortic. 1185: 11-20.
Lorenzi, H. & Matons, F. (2008). Plantas Medicinais: Nativas e Exóticas. 2. ed. Nova Odessa: Instituto Plantarum, 544 p.
Magnitskiy, S. & Plaza, G. (2007) Fisiología de semillas recalcitrantes de árboles tropicales. Agronomía Colombiana. 25 (1): 96-103.
Nkang, A. (2002). Carbohydrate composition during seed development and germination in two sub-tropical rainforest tree species (Erythrina caffra and Guilfoylia monostylis). J. Plant Physiol. 159 (5): 473-483.
Patel, K., Gadewar, M., Tripathi, R., Prasad, S., Patel, D. (2012). A review on medicinal importance, pharmacological activity and bioanalytical aspects of beta-carboline alkaloid “Harmine”. Asian Pacific Journal of Tropical Biomedicine. 2 (8): 660-664.
Pérez, A.E. (1996). Plantas útiles de Colombia. Quinta edición. Fondo FEN Colombia. Bogotá, D.C., Colombia. 831 p.
Rao, N.K., Hanson, J., Dulloo, E.M., Ghosh, K., Nowell, D., Larinde, M. (2007). Manual para el manejo de semillas en bancos de germoplasma No.8. Biodiversity International. Roma, Italia. 165 p.
Ribeiro, S. (2014). Propagação in vitro e ex vitro de Symplocos uniflora (pohl.) Benth. (Symplocaceae). (Tesis pósgraduação em agrobiologia) Universidade Federal de Santa Maria, Centro de ciências naturais e exatas. Santa Maria. Brasil. 55 p.
Rojas-Aréchiga, M., Casas, A., Vázquez-Yanes C. (2001). Seed germination of wild and cultivated Stenocereus stellatus (Cactaceae) from the TehuacanCuicatlan Valley, Central Mexico. Journal of Arid Environments. 49: 279-287.
Souza, V. & Lorenzi, H. (2005). Botánica sistemática: guía ilustrada para identificación de familias angiospermas de la flora brasileña, basado en APGII. 1 ed. Nova Odessa: Instituto Plantarum. 640 p.
Suárez, D., Fernández, J., Melgarejo, L. (2011). Efecto de la luz y del ácido giberélico (AG3) en la germinación de Monthostachys mollis KUNTH. GRISEB (LABIATAE). Acta biológica Colombiana. 16 (2): 149-154.
Toro, J.L. & Vanegas, G.L. (2002). Flora de los páramos y bosques altoandinos del noroccidente medio de Antioquia. Corporación Autónoma Regional del Centro de Antioquia, Corantioquia. 1ª ed. Editorial Multimpresos, Medellín- Colombia. p. 180.
Tompsett, P.B. & Pritchard, H.W. (1998). The effect of chilling and moisture stress on the germination, desiccation tolerance and longevity of Aesculus hippocastanum L. seeds. Annals of Botany (Oxford, United Kingdom). 82: 249-261.
Viswanath, S., Singh, R., Thapliyal, R. (2002). Seed germination patterns in a himalayan moist temperate forest. Tropical Ecology. 43 (2): 265-273.
Zhang, Y., Du, X., Zhang, Q., Gao, X., Su, Z., (2005). Fitness analysis of seed and vegetative reproduction of clonal tree Symplocos laurina. Ying Yong Sheng Tai Xue Bao. 16 (9):1687-92.
Declaration of originality and transfer author's rights
The authors declare:
- The published data and reference materials have been duly identified with their respective credits and have been included in the bibliographic notes and citations that have been so identified and that should it be required, I have all releases and permissions from any copyrighted material.
- All material presented is free from any copyright and that I accept full legal responsibility for any legal claims relating to copyrighted intellectual property, fully exonerating from responsibility the Revista de la Academia Colombiana de Ciencias Exactas, Físicas y Naturales.
- This work is unpublished and will not be sent to any other journal while waiting for the editorial decision of this journal. I declare that there is no conflict of interest in this manuscript.
- In case of publication of this article, all author´s rights are transferred to the Revista de la Academia Colombiana de Ciencias Exactas, Físicas y Naturales, and so cannot be reproduced in any form without the express permission of it.
- By means of this document, if the article is accepted for publication by the Revista de la Academia Colombiana de Ciencias Exactas, Físicas y Naturales, the Revista assumes the right to edit and publish the articles in national and international indices or data bases for academic and scientific use in paper, electronic, CD-ROM, internet form either of the complete text or any other known form known or to be known and non-commercial, respecting the rights of the authors.
Transfer of author rights
In case the article is approved for publication, the main author in representation of himself and his co-authors or the main author and his co-authors must cede the author rights of the corresponding article to the Revista de la Academia Colombiana de Ciencias Exactas, Físicas y Naturales, except in the following cases:
The authors and co-authors will retain the right to revise, adapt, prepare derived works, oral presentations, and distribution to some colleagues of reprints of their own published work, if the corresponding credit is given to the Revista de la Academia Colombiana de Ciencias Exactas, Físicas y Naturales. It is also permissible to publish the title of the work, summary, tables, and figures of the work in the corresponding web sites of the authors or their employers, also giving credit to the Revista.
If the work has been realized under contract, the author’s employer has the right to revise, adapt, prepare derivative works, reproduce, or distribute in hard copy the published work, in a secure manner and for the exclusive use of his employees.
If the Revista de la Academia Colombiana de Ciencias Exactas, Físicas y Naturales were approached for permission by a third party for using, printing, or publishing specifically articles already published, the Revista must obtain the express permission of the author and co-authors of the work or of the employer except for use in classrooms, libraries, or reprinted in a collective work. The Revista de la Academia Colombiana de Ciencias Exactas, Físicas y Naturales reserves the possible use in its front cover of figures submitted with the manuscripts.
No other right, other than the author’s right, can be claimed by the Revista de la Academia Colombiana de Ciencias Exactas, Físicas y Naturales.