Characterization of the causal agent of the deformation of the fruits of the grape (Vitis vinifera L.) var. Red Globe, in La Unión, Valle del Cauca, Colombia
Portada 43 (167) 2019
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López-Zapata, S. P., Castaño-Zapata, J., Arango-Isaza, R., & Vásquez-Barajas, D. A. (2019). Characterization of the causal agent of the deformation of the fruits of the grape (Vitis vinifera L.) var. Red Globe, in La Unión, Valle del Cauca, Colombia. Revista De La Academia Colombiana De Ciencias Exactas, Físicas Y Naturales, 43(167), 241–249. https://doi.org/10.18257/raccefyn.844

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Abstract

In recent years, a disease characterized by the necrosis of the grape berries (Vitis viinifera L.) has appeared on the Red Globe variety cultivated in vineyards in the municipality of La Unión, Valle del Cauca, Colombia, resulting in loss of yield and quality. To identify the etiology of the disease, fruits showing the typical symptoms of discoloration and subsequent necrosis were collected and processed. With high frecuency was found a fungus that was subjected to pathogenicity tests and morphological characterization by light and electron microscopy (ESEM). Koch´s postulates were fulfilled by the inoculation of a conidial suspension of 1x106 conidia per milliliter of water on healthy berries of the same variety. After a week, symptoms similar to those observed in the field began to be observed. The search for similitudes with BLAST showed that the sequences of ITS had a 100% identity with Colletotrichum aenigma and C. siamense belonging to the complex of species of Colletotrichum gloeosporioides, which provides useful information to understand the disease of the grape berries and to design management  trategies to control it. © 2019. Acad. Colomb. Cienc. Ex. Fis. Nat.

https://doi.org/10.18257/raccefyn.844
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References

Afanador-Kafuri, L., Minz, D., Maymon, M., Freeman, S. (2003). Characterization of Colletotrichum isolates from tamarillo, Passiflora, and mango in Colombia and identification of a unique species from the genus. Phytopathology. 93(5):579- 587. Doi.org/10.1094/PHYTO.2003.93.5.579

Agrios, G.N. (2005). Plant pathology, 5th ed. Academic, New York. 952 p.

Agronet. (2018). Estadísticas de la producción de uva en Colombia. Fecha de consulta: 26 de julio, 2018. Disponible en: http://www.agronet.gov.co/estadistica/Paginas/default.aspx

Baroncelli, R., Sreenivasaprasad S., Lane, C.R., Thon, M.R., Sukno, S.A. (2014). First report of Colletotrichum acutatum sensu lato (Colletotrichum godetiae) causing anthracnose on grapevine (Vitis vinifera) in the United Kingdom. New Disease Reports. 29: 26. Doi: 10.5197/j.2044-0588.2014.029.026

Cai, L., Hyde, K.D., Taylor, P.W.J., Weir, B.S., Waller, J., Abang, M.M., Zhang, J.Z., Yang, Y.L., Phoulivong, S., Liu, Z.Y., Prihastuti, H., Shivas, R.G., McKenzie,E.H.C., Johnston, P.R. (2009). A polyphasic approach for studying Colletotrichum. Fungal Diversity. 39: 183-204.

Chowdappa, P., Reddy, G.S., Kumar, A., Rao, B.M., Rawal, R.D. (2009). Morphological and molecular characterization of Colletotrichum species causing anthracnose of grape in India. The Asian and Australasian Journal of Plant Science and Biotechnology. 3 (1): 71-77.

Daykin, M. E., Milholland, R. D. (1984a). Histopathology of ripe rot caused by Colletotrichum gloeosporioides on muscadine grape. Phytopathology. 74 (11): 1339-1341.

Daykin, M. E., Milholland, R. D. (1984b). Ripe rot of Muscadine grape caused by Colletotrichum gloeosporioides and its control. Phytopathology. 74: 710-714. Doi: 10.1094/Phyto-74-710.

Faz, R., Madero, E., Lagarda, A., Preciado, P., Ávila, C. (2013). Producción y calidad de la uva de mesa de la variedad Red Globe (Vitis vinifera L.) sobre diferentes porta injertos y densidades de población. Agrofaz. 13 (3):105-110. Disponible desde internet en: https://dialnet.unirioja.es/servlet/articulo?codigo=5530959

Ferreira, T., Rasband, W. (2012). ImageJ user guide/IJ 1.46r. Bioimage Informatics. http://rsb.info.nih.gov/ij/docs/guide/user-guide.pdf.

Freeman, S., Katan, T., Shabi, E. (1998). Characterization of Colletotrichum species responsible for Anthracnose diseases of various fruits. Plant Disease. 82 (6): 596-605.

Gil, L.F., Varzea, V.M., Do Ceu Silva, M. (2002). La enfermedad de las cerezas del café –CBD- causada por Colletotrichum kahawae. Avances Técnicos 298. Cenicafé. p. 8.

Greer, L.A., Harper, J.D.I., Savocchia, S., Samuelian, S.K., Steel, S.C. (2011). Ripe rot of South-eastern Australian wine grapes is caused by two species of Colletotrichum: C. acutatum and C. gloeosporioides with differences in infection and fungicide sensitivity. Australian Journal of Grape and Wine Research. 17: 123-128. Doi: 10.1111/j.1755-0238.2011.00143.x

Harp, T., Pernezny, K., Lewis, M., Miller, S., Kuhn, P., Datnoff, L. (2008). The etiology of recent pepper anthracnose outbreaks in Florida. Crop Protection. 27 (10):1380-1384. Doi: 10.1016/j.cropro.2008.05.006

Hernández, J., Trujillo, Y., Durán, D. (2011). Contenido fenólico e identificación de levaduras de importancia vínica de la uva Isabela (Vitis labrusca), procedente de Villa del Rosario (Norte de Santander). Revista de la Facultad de Química Farmacéutica. 18 (1): 17-25.

Huang, Y.Q., Zang, C.Q., Li, L.X., Liang, C.H., Bai, Y.J., Xie, J.H. (2016). Assessing the genetic diversity of grape ripe rot pathogen Colletotrichum using SRAP markers. Mycosphere. 7 (8): 1103-1110. Doi: 10.5943/mycosphere/si/2c/3

Jiang, J., Zhai, H., Li, H., Wang, Z., Chen, Y., Hong, N., Wang, G., Chofong, G.N., Xu, W. (2014). Identification and characterization of Colletotrichum fructicola causing black spots on young fruits related

o bitter rot of pear (Pyrus bretschneideri Rehd.) in China. Crop Protection. 58: 41-48. Doi: 10.1016/j.cropro.2014.01.003.

Kimura, M. (1980). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J. Mol. Evol. 16: 111-120.

Kummuang, N., Diehl, S. V., Smith, B. J., Graves, C.H. Jr. (1996). Muscadine grape berry rot diseases in Mississippi: Disease epidemiology and crop reduction. Plant Disease. 80 (3): 244-247.

Lewis, M. L., Nava, C., Miller, S. A. (2004). Identification and management of Colletotrichum acutatum on immature bell peppers. Plant Disease. 88 (11):1198-1204. Doi: 10.1094/PDIS.2004.88.11.1198

Lorenz, D.H., Eichorn, K.W., Bleiholder, H., Klose, R., Meier U., Weber, E. (1994). Phänologische Entwicklungsstadien der Weinrebe (Vitis vinifera L. ssp. vinifera). Codierung und Beschreibung nach der erweiterten BBCH-Skala, Vitic. Enol. Sci. 49: 66-70.

Onesti, G. (2015). Studies on inoculum dynamics of Guignardia bidwellii, causal agent of grape black rot. Tesis doctoral. Università Cattolica del Sacro Cuore, Piacenza. Italia. P. 170.

Peng, L. J., Sun, T., Yang, Y., Cai, L., Hyde, K. D., Bahkali, A. H., Liu, Z. Y. (2013). Colletotrichum species on grape in Guizhou and Yunnan provinces, China, Mycoscience. 54 (1): 29-41. Doi: 10.1016/j.myc.2012.07.006

Peres, N., Timmer, L., Adaskaveg, J., Correl, J. (2005). Lifestyles of Colletotrichum acutatum. Plant Disease. 89 (8):784-796. Doi: 10.1094/PD-89-0784.

Phoulivong, S., Cai, L., Chen, H., Mckenzie, E.H.C., Abdelsalam, K., Chukeatirote, E., Hyde, K. D. (2010). Colletotrichum gloeosporioides is not a common pathogen on tropical fruits. Fungal Diversity. 44 (1): 33-43. Doi: 10.1007/s13225-010-0046-0.

Prakash, O., Misra, A.K., Pandey, B.K. (1996). Anthracnose disease of tropical and subtropical fruits. Disease Scenario in Crop Plants. p. 1-27. En: Vol. 1. Fruits and Vegetables. V.P. Agnihotri, O. Prakash, R. Kishun and A.K, Misra. (Editores). International Books and Periodical Supplies Service. Pitampura, Delhi, India.

Prihastuti, H., Mckenzie, E.H.C., Hyde, K.D., Cai, L., Chen, H. (2009). Characterization of Colletotrichum species associated with coffee berries in northern Thailand. Fungal Diversity. 39: 89-109.

Riascos, R. (2011). Caracterización etiológica de la roña de la gulupa (Passiflora edulis Sims.) en la región de Sumapaz, Cundinamarca. Tesis para optar el título de Magister en Ciencias Agrarias con énfasis en Fitopatología. Universidad Nacional de Colombia. Bogotá. Colombia. p. 96.

Reader, U., Broda, P. (1985). Rapid preparation of DNA from filamentous fungi. Letters in Applied Microbiology. 1: 17-20.

Rhaiem, A., Taylor, P. W. (2016). Colletotrichum gloeosporioides associated with anthracnose symptoms on citrus, a new report for Tunisia. European Journal of Plant Pathology. 146 (1): 219-224.

Ruvishika, S. Jayawardena, Wei Zhang, Mei Liu, Sajeewa, S.N., Maharachchikumbura, Ying Zhou, JinBao Huang, Somrudee Nilthong, ZhongYue Wang, XingHong Li, JiYe Yan, Kevin D. Hyde. (2015). Identification and characterization of Pestalotiopsis-like fungi related to grapevine diseases in China. Fungal Biology. 119 (5): 348-361. Doi: 10.1016/j.funbio.2014.11.001

Saitou, N., Nei, M. (1987). The neighbor-joining method: A new method for reconstructing phylogenetic trees. Molecular Biology and Evolution. 4 (4): 406-425.

Saldarriaga, A. (2006). Caracterización del agente causante de la antracnosis en tomate de árbol, manzana y mora. Manizales. Tesis de Magíster en Fitopatología. Facultad de Ciencias Agropecuarias. Universidad de Caldas. p. 191.

Santos, R. F., Ciampi‐Guillardi, M., Amorim, L., Massola, N. S., Spósito, M. B. (2018). Aetiology of anthracnose on grapevine shoots in Brazil. Plant Pathol. 67: 692-706. Doi: 10.1111/ppa.12756.

Sawant, I.S., Narkar, S.P., Shetty, D.S. Upadhyay, A., Sawant, S.D. (2012). Emergence of Colletotrichum gloeosporioides sensu lato as the dominant pathogen of anthracnose disease of grapes in India as evidenced by cultural, morphological and molecular data. Australasian Plant Pathology. 41 (5):493-504. Doi: 10.1007/s13313-012-0143-5

Shiraishi, M., Yamada, M., Mitani, N., Ueno, T., Nakaune, R., Nakano, M. (2006). Rapid screening assay for Ripe Rot resistance in grape cultivars. Journal of Japanese Society for Horticultural Science. 75 (3): 264-266. Doi: 10.2503/jjshs.75.264

Shiraishi, M., Koide, M., Itamura, H., Yamada, M., Mitani, M., Ueno, T., Nakaune, R., Nakano, M. (2007). Screening for resistance to ripe rot caused by Colletotrichum acutatum in grape germ plasm. Vitis. 46 (4): 196-200.

Sung, K.H, Wang, G.K, Hae, G.K., Kyung, J.C. (2008). Morphological variations, genetic diversity and pathogenicity of Colletotrichum species causing grape ripe rot in Korea. The Plant Pathology Journal. 24 (3): 269-278. Doi: 10.5423/PPJ.2008.24.3.269

Suzaki, K. (2011). Improved method to induce sporulation of Colletotrichum gloeosporioides, causal fungus of grape ripe rot. General Plant Pathology. 77 (2): 81-84. Doi:10.1007/s10327-011-0296-z

Tamura, K., Stecher, G., Peterson, D., Filipski, A., Kumar, S. (2013). MEGA6: molecular evolutionary genetics analysis version 6.0. Molecular Biology and Evolution. 30 (12):2725-2729.

Weir, B.S., Johnston, P.R., Damm, U. (2012). The Colletotrichum gloeosporioides species complex. Studies in Mycology. 73(1): 115-180. Doi: 10.3114/sim0011

Weising, K., Beyermann, K., Ramser, J., Hahl, G. (1991). Plant DNA fingerprinting with radioactive and digoxigenated oligonucleotide probes complementary to simple repetitive DNA sequences. Electrophoresis. 12: 159-169.

White, T. J., Bruns, T., Lee, S. J. W. T., Taylor, J. W. (1990). Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. PCR Protocols: A Guide to Methods and Applications. 18 (1): 315-322.

Whitelaw-Weckert, M. A., Curtin, S. J., Huang, R., Steel, C. C., Blanchard, C. L., Roffey, P. E. (2007). Phylogenetic relationships and pathogenicity of Colletotrichum acutatum isolates from grape in subtropical Australia. Plant Pathology. 56 (3): 448-463. Doi: 10.1111/j.1365-3059.2007.01569.x

Yan, J.Y., Jayawardena, M.M.R.S., Goonasekara, I.D., Wang, Y. (2014). Diverse species of Colletotrichum associated with grapevine anthracnose in China. Fungal Diversity. 71 (1): 233-246. Doi: 10.1007/s13225-014-0310-9

Zhang, P.F., Zhai, L.F., Zhang, X.K. Huang, X.Z., Hong, N., Xu, W.X., Wang, G.P. (2015). Characterization of Colletotrichum fructicola, a new causal agent of leaf black spot disease of sandy pear (Pyrus pyrifolia). European Journal of Plant Pathology. 143 (4): 651-662. Doi: 10.1007/s10658-015-0715-7

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