OBTENCIÓN Y CARACTERIZACIÓN DE UN NOVEL ALERGENO MEDIANTE LA TECNOLOGÍA DEL DNA RECOMBINANTE

Leonardo  Puerta

doi:10.18257/raccefyn.25(94).2001.2716

Vol. 25 No. 94 (2001), Natural Sciences

Vol. 25 No. 94 (2001)

ACQUISITION AND CHARACTERIZATION OF A NEW ALIERGEN USING RECOMBINANT DNA TECHNOLOGY

Natural Sciences

https://doi.org/10.18257/raccefyn.25(94).2001.2716

Published 2024-08-01

Leonardo Puerta⁺⁻

Leonardo Puerta

Instituto de Investigaciones Inmunológicas. Universidad de Cartagena, Apartado Aéreo 4610 Cartagena, Colombia

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How to Cite

Puerta, L. (2024). ACQUISITION AND CHARACTERIZATION OF A NEW ALIERGEN USING RECOMBINANT DNA TECHNOLOGY. Revista De La Academia Colombiana De Ciencias Exactas, Físicas Y Naturales, 25(94), 77–87. https://doi.org/10.18257/raccefyn.25(94).2001.2716

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Abstract

Production and characterization of allergens are important to study their properties and improve the handling of allergic diseases. By using DNA recombinant technology a novel allergen, Blot 13, was identified and added to the list of characterized allergens from the World Heat

https://doi.org/10.18257/raccefyn.25(94).2001.2716

Keywords

recornbinant allergens | allergy | fatty acid binding roteins | lgE | rnites

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References

Creg JM, Vedvick TS, & Raschke WC. 1993 Rccent advanccs in the exprcssion of forcing genes in Pichia pastoris. Bioffechnology 1993;11:905-910.

Erlksson T, Whltley P, Johansson E, van Hage-Hamsten M, & Gatvelln G. 1999. Identificación and Characterization of two allergens from the dust mite Acarus siro, homologous with fatty acid-binding protcins. Int Arch Allergy Immunol 119:275- 281.

Erlkson T, Rasool O, Whltley P, Cramerl R, & van Hage-Hamsten M. 2000. Cloning of two allergens from the dust mite lepidoglyphus destructor using phage display technology.J Allergy Clin lmmunol 105: S229

Fernández-Caldas E, Puerta L, Mercado D, Lockey RF, & Caraballo L. J993. Mite fauna, Dcr p 1, Dcr f 1, and Blomia tropicalis allergens levels in a tropical enverionment. Clin Exp Allergy 23:292-297.

Guex N, & Peltsch MC. 1997. SWISS-MODEL and the Swiss PdbViewer:An environment for comparative modelinling. Electrophorcsis 18:2714-2723.

ameson DA & Wolf H. 1988 The antigenic indcx: a novel algorithm for prcdicting antigenic determinants Cabios 4: 181-186.

Kennedy M W, Drass A, McCruden DA., Prlce NC, Kelly SM, & Cooper A. 1995. The ABA-1 allergen of the parasitic nematode Ascaris suum: Fatty acid and rctinoid binding function and structurcs characterization. Biochemistry. 34:6700-6710.

Laskowsld RA, MacArthur MW,Moss DS, & Thortnton JM. 1993. PROCHECK: A program to check the stereochemical quality of protcin structurcs. J Appl Cryst:26:283-291.

Lynch NR, Thomas WR, Garcla NM, DI Prisco MC, Puccio FA, Lopez R, Hazell L, Shen HD, Llnd KL, & Chua KY.1997 Biological activity of rccombinant Dcr p 2, der p 5 and Dcr p 7 allergens of the house-dust mite Dermatophagoides pteronyssinus. Int Arch Allergy lmmunol 114:59-67.

Murray V. 1989. Improved double-stranded DNA sequencing using the linear polymerase chain reaction. Nucleic Acids Res. 17:8889

Provencher SW, & Gloclmer J. 1981. Estimation of globular protcin secondary structurc from circular dichroism, Biochemistry 20: 33-37.

Puerta L, Fernández-Caldas E, Lockey RF, & Caraballo L.1993. gE to six mites species in Cartagena Colombia. J. lnvest Allergology Clin lmmunol .3: 198-204.

Puerta L, Fernández-Caldas E, Mercado D, Lockey R, & Caraballo L. 1996. Sequential determinations of Blomia tropicalis allergens in mattrcss and floor dust samples in a tropical city. J. Allergy Clin lmmunol. 97:689-691.

Puerta L, Caraballo L, Fernández-Caldas E, Avjloglu A, Marsh D, Lockey R, & Dao M. 1996. Nucleotide scquence analysis of a cDNA coding for a Blomia tropicalis allergen. Allergy Clin Immunol. 98:932-937.

Puerta L, Malcolm K, Jlmenez S, & Caraballo L. 1999. Structural and Ligand Binding Analysis of Recombinant Blot 13 allergen from Blomia tropicalis mitcs, a Fatty Acid Binding Protein Int Arch Alergy lmmunól.:119:181-184.

Puerta L, Jimenez S, Lagares A, & Caraballo L, 1999. High-level exprcssion of the Blomia tropicalis allergen Blo t 13 in Pichia pastoris. J. Allergy Clin Immnol;103:Sl85)

Rost D, & Sander C. 1994. Combining evolutionary information and natural networlc to prcdict pritein secondary structure. Protcins. 19:55-72

Sander C, & Schnelder R. 1991. Database of homology-derived proteins structure and the structure meaning of sequence alignment, Proteins. 9, 56-68.

Salcedo G, Diip:-Perales A. & Sanchez-Monge R.1999. Fruit allergy: plant defence proteins as novel potential panallergens. Clin Exp Allergy. 29:1158-1160.

Sambrook J, Frltsch F, & Maniatis T. 1989. Molecular cloning: a laboatory manual. Cold Spring Harbor:Cold Spring Harbor Laboatory 2:67-98.

Sanjuan H, Puerta L, & Caraballo L. 1998. Expression of a Blonúa tropicalis allcrgen in the yest Pichia pastoris. J Allergy Clin Immnol 101:S155.

Thomas WR, & Smlth W. 1998. An updatc on allergens. House-dust mite allergens. Allergy 1998;53:821-832.

Tsal J, Wu H, Shen H, Hsu E, & Wang AS. 1998. Sensitization to Blomia tropicalis among asthmatic patients in Taiwan. Int Arch Allergy lmmunol 115:144-149.

Sakar G, & Sommer S. 1990. The "mega" Primer method of site directed mutagenesis. Biotechhnoiques 8:404-407.

Valenta R, Hayek D, Selberler S, Bugajaka-Schretter, Nlederberger V, Twardos:r.A, Natter S, Vangellsta L, Pastore A, Spltzauer S, & Kraft D. 1998. Calcium-Binding Allergens: From Plants to man. Int Arch Allergy Immunol. 117:160-166.

Valenta R, Lldholm J, Nlederberger V, Hayek D, Kraft D, & Gronlund H. 1999 The recombinant allergen-based concept of component-reolved diagnostics and immunotherapy (CRD and CRIT) Clin Exp Allergy 29:896-904.

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