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Abstract
Calcium plays a fundamental role in the development of Plasmodium falciparum, the intracellular parasite that causes malaria. With the purpose of understanding the mechanism by which calcium acts in this parasite, Calcium-binding proteins were detected in this organism. The combined use of the metachromatic dye “Stains-all” and the 45Ca Overlay assay allowed the identification, in mature parasites, of 9 calcium-binding proteins, 6 of which seem to be different from any reported calcium- binding protein. Additionally, it was determined that the combined use of these techniques can be useful for the detection and purification of calcium-binding proteins.
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