Cómo citar
Descargas
Métricas Alternativas
Dimensions
Resumen
La nutrición es un determinante de la respuesta inmune, y el déficit de proteína y calorías tienen efectos deletéreos en los sistemas de defensa de un organismo. La relación entre nutrición, migración y resistencia a infecciones es muy compleja. La quimiotaxis, o migración celular dirigida, es un fenómeno fundamental en procesos fisiológicos y patológicos. La quimioquina CXCL12 o factor derivado de células estromales-1 (SDF-1) y su receptor CXCR4 son reguladores de la hematopoyesis, la iniciación de la respuesta inmune adaptativa y la vigilancia inmunológica en tejidos periféricos. No se conoce el papel regulador de la nutrición sobre la expresión del receptor CXCR4 y sus implicaciones en la respuesta inmune. En este trabajo se examinó la expresión del receptor CXCR4 en esplenocitos y timocitos de ratón bajo restricción nutricional y frente a un reto con un agente infeccioso, Listeria monocytogenes. El análisis de la distribución de las diferentes subpoblaciones linfoides y la expresión del receptor CXCR4 se realizó el día 3 post-infección mediante citometría de flujo. Los resultados mostraron que el receptor CXCR4 se expresa con mayor abundancia en timocitos que en esplenocitos. La infección bacteriana indujo cambios significativos en los niveles de expresión del receptor y se observó que el perfil de expresión es sensible a los cambios en la ingesta de proteína.
Palabras clave
Citas
Aboumrad, E., Madec, A. M. & Thivolet, C., 2007. The CXCR4/CXCL12 (SDF-1) signalling pathway protects non-obese diabetic mouse from autoimmune diabetes. Clin Exp Immunol. 148 (3): 432-9.
Baetz, A. L. & Wesley, I. V., 1995. Detection of anti-listeriolysin O in dairy cattle experimentally infected with Listeria monocytogenes. J Vet Diagn Invest. 7 (1): 82-6.
Baggiolini, M., 1998. Chemokines and leukocyte traffic. Nature. 392(6676): 565-8.
Barsig, J., Flesch, I. E. & Kaufmann, S. H., 1998. Macrophages and hepatocytic cells as chemokine producers in murine listeriosis. Immunobiology. 199 (1): 87-104.
Berche, P., Reich, K. A., Bonnichon, M., Beretti, J. L., Geoffroy, C., Raveneau, J., Cossart, P., Gaillard, J. L., Geslin, P., Kreis, H. & et al., 1990. Detection of anti-listeriolysin O for serodiagnosis of human listeriosis. Lancet. 335 (8690): 624-7.
Bhunia, A. K., 1997. Antibodies to Listeria monocytogenes. Crit Rev Microbiol. 23 (2): 77-107.
Carrero, J. A., Calderon, B. & Unanue, E. R., 2006. Lymphocytes are detrimental during the early innate immune response against Listeria monocytogenes. J Exp Med. 203 (4): 933-40.
Cavalcanti, D. M., Lotufo, C. M., Borelli, P., Tavassi, A. M., Pereira, A. L., Markus, R. P. & Farsky, S. H., 2006. Adrenal deficiency alters mechanisms of neutrophil mobilization. Mol Cell Endocrinol. 249 (1-2): 32-9.
Chinen, J. & Shearer, W. T., 2008. 6. Secondary immunodeficiencies, including HIV infection. J Allergy Clin Immunol. 121 (2 Suppl): S388-92; quiz S417.
Cook, D. N., Smithies, O., Strieter, R. M., Frelinger, J. A. & Serody, J. S., 1999. CD8+ T cells are a biologically relevant source of macrophage inflammatory protein-1 alpha in vivo. J Immunol. 162 (9): 5423-8.
Derek, M., 2005. Malnutrition and infection. 33 (3): 14-16. Desmetz, C., Lin, Y. L., Mettling, C., Portales, P., Noel, D., Clot, J., Jorgensen, C. & Corbeau, P., 2007. Cell surface CCR5 density determines the intensity of T cell migration towards rheumatoid arthritis synoviocytes. Clin Immunol. 123 (2): 148-54.
Ebert, L. M., Schaerli, P. & Moser, B., 2005. Chemokine-mediated control of T cell traffic in lymphoid and peripheral tissues. Mol Immunol. 42 (7):799-809.
Edelson, B. T., Cossart, P. & Unanue, E. R., 1999. Cutting edge: paradigm revisited: antibody provides resistance to Listeria infection. J Immunol. 163 (8): 4087-90.
Edelson, B. T. & Unanue, E. R., 2000. Immunity to Listeria infection. Curr Opin Immunol. 12 (4): 425-31.
Edelson, B. T. & Unanue, E. R., 2001. Intracellular antibody neutralizes Listeria growth. Immunity. 14 (5): 503-12.
El-Hodhod, M. A., Nassar, M. F., Zaki, M. M. & Moustafa, A., 2005. Apoptotic changes in lymphocytes of protein energy malnutrition patients. Nutrition Research. 25 (1): 21-29.
Foster, M. P., Jensen, E. R., Montecino-Rodriguez, E., Leathers, H., Horseman, N. & Dorshkind, K., 2000. Humoral and cell mediated immunity in mice with genetic deficiencies of prolactin, growth hormone, insulin-like growth factor-I, and thyroid hormone. Clin Immunol. 96 (2): 140-9.
Franz, C. M., Jones, G. E. & Ridley, A. J., 2002. Cell migration in development and disease. Dev Cell. 2 (2): 153-8.
Gentschev, I., Sokolovic, Z., Kohler, S., Krohne, G. F., Hof, H., Wagner, J. & Goebel, W., 1992. Identification of p60 antibodies in human sera and presentation of this listerial antigen on the surface of attenuated salmonellae by the HlyB-HlyD secretion system. Infect Immun. 60 (12): 5091-8.
Ghosh, S., Preet, A., Groopman, J. E. & Ganju, R. K., 2006. Cannabinoid receptor CB2 modulates the CXCL12/CXCR4-mediated chemotaxis of T lymphocytes. Mol Immunol. 43(14): 2169-79.
Grenningloh, R., Darji, A., Wehland, J., Chakraborty, T. & Weiss, S., 1997. Listeriolysin and IrpA are major protein targets of the human humoral response against Listeria monocytogenes. Infect Immun. 65 (9): 3976-80.
Jin, T. & Hereld, D., 2006. Moving toward understanding eukaryotic chemotaxis. Eur J Cell Biol. 85 (9-10): 905-13.
Landgraf, M. A., Martinez, L. L., Rastelli, V. M., Franco Mdo, C., Soto-Suazo, M., Tostes Rde, C., Carvalho, M. H., Nigro, D. & Fortes, Z. B., 2005. Intrauterine undernutrition in rats interferes with leukocyte migration, decreasing adhesion molecule expression in leukocytes and endothelial cells. J Nutr. 135 (6): 1480-5.
Landgraf, M. A., Tostes Rde, C., Borelli, P., Zorn, T. M., Nigro, D., Carvalho, M. H. & Fortes, Z. B., 2007. Mechanisms involved in the reduced leukocyte migration in intrauterine undernourishment. Nutrition. 23 (2): 145-56.
Lin, J. S. & Wu-Hsieh, B. A., 2004. Functional T cells in primary immune response to histoplasmosis. Int Immunol. 16 (11): 1663-73.
Mackaness, G. B., 1971. Resistance to intracellular infection. J Infect Dis. 123 (4): 439-45.
Matsuzaki, G., Vordermeier, H. M., Hashimoto, A., Nomoto, K. & Ivanyi, J., 1999. The role of B cells in the establishment of T cell response in mice infected with an intracellular bacteria, Listeria monocytogenes. Cell Immunol. 194 (2): 178-85.
Menon, A., Shroyer, M. L., Wampler, J. L., Chawan, C. B. & Bhunia, A. K., 2003. In vitro study of Listeria monocytogenes infection to murine primary and human transformed B cells. Comp Immunol Microbiol Infect Dis. 26 (3): 157-74.
Merrick, J. C., Edelson, B. T., Bhardwaj, V., Swanson, P. E. & Unanue, E. R., 1997. Lymphocyte apoptosis during early phase of Listeria infection in mice. Am J Pathol. 151 (3): 785-92.
Mombaerts, P., Arnoldi, J., Russ, F., Tonegawa, S. & Kaufmann, S. H., 1993. Different roles of alpha beta and gamma delta T cells in immunity against an intracellular bacterial pathogen. Nature. 365 (6441): 53-6.
Moser, B., Wolf, M., Walz, A. & Loetscher, P., 2004. Chemokines: multiple levels of leukocyte migration control. Trends Immunol. 25 (2): 75-84.
Murciano, C., Villamon, E., O’Connor, J. E., Gozalbo, D. & Gil, M. L., 2006. Killed Candida albicans yeasts and hyphae inhibit gamma interferon release by murine natural killer cells. Infect Immun. 74 (2): 1403-6.
Najera, O., Gonzalez, C., Toledo, G., Lopez, L. & Ortiz, R., 2004. Flow cytometry study of lymphocyte subsets in malnourished and well-nourished children with bacterial infections. Clin Diagn Lab Immunol. 11 (3): 577-80.
Naranjo, W. M., Yakar, S., Sanchez-Gomez, M., Perez, A. U., Setser, J. & D, LeRoith., 2002. Protein calorie restriction affects nonhepatic IGF-I production and the lymphoid system: studies using the liver-specific IGF-I gene-deleted mouse model. Endocrinology. 143 (6): 2233-41.
Norine, J. D. & John, R. S., 2003. Role of neutrophils in decreased resistance of protein-malnourished mice to Candida albicans. 23 (7): 945-958.
North, R. J. & Conlan, J. W., 1998. Immunity to Listeria monocytogenes. Chem Immunol. 70: 1-20.
Pamer, E. G., Sijts, A. J., Villanueva, M. S., Busch, D. H. & Vijh, S., 1997. MHC class I antigen processing of Listeria monocytogenes proteins: implications for dominant and subdominant CTL responses. Immunol Rev. 158: 129-36.
Pease, J. E. & Williams, T. J., 2006. Chemokines and their receptors in allergic disease. J Allergy Clin Immunol. 118 (2): 305-18; quiz 319-20.
Peel, M., Donachie, W. & Shaw, A., 1988. Temperature-dependent expression of flagella of Listeria monocytogenes studied by electron microscopy, SDS-PAGE and western blotting. J Gen Microbiol. 134 (8): 2171-8.
Rossi, D. & Zlotnik, A., 2000. The biology of chemokines and their receptors. Annu Rev Immunol. 18: 217-42.
Sambrook, J., Fritsch, E. F. & Maniatis, T., 1989. Molecular Cloning. A Laboratory Manual C. S. H. L. Press. NY.
Schaible, U. E., Collins, H. L. & Kaufmann, S. H., 1999. Confrontation between intracellular bacteria and the immune system. Adv Immunol. 71:267-377.
Tarrant, T. K. & Patel, D. D., 2006. Chemokines and leukocyte trafficking in rheumatoid arthritis. Pathophysiology. 13 (1):1-14.
Welniak, L. A., Karas, M., Yakar, S., Anver, M. R., Murphy, W. J. & LeRoith, D., 2004. Effects of organ-specific loss of insulin like growth factor-I production on murine hematopoiesis. Biol Blood Marrow Transplant. 10 (1): 32-9.
Yamaguchi, H. & Condeelis, J., 2007. Regulation of the actin cytoskeleton in cancer cell migration and invasion. Biochim Biophys Acta. 1773 (5):642-52.
Yoshinouchi, T., Naniwa, T., Shimizu, S., Ohtsuki, Y., Fujita, J., Sato, S., Eimoto, T. & Ueda, R., 2007. Expression of chemokine receptors CXCR3 and CCR4 in lymphocytes of idiopathic nonspecific interstitial pneumonia. Respir Med. 101(6): 1258-64.
Esta obra está bajo una licencia internacional Creative Commons Atribución-NoComercial-SinDerivadas 4.0.
Derechos de autor 2023 Revista de la Academia Colombiana de Ciencias Exactas, Físicas y Naturales