How to Cite
Downloads
Métricas Alternativas
Dimensions
Abstract
Given the pharmacological importance of tropane alkaloids and the possibilityof producing them from plant tissue cultures of plant species such as Brugmansia candida, the main goal of the present paper was to evaluate the alkaloid production of root cultures developed ina bioreactor. We set up a bioreaction system consisting of two units, one forbiomass growth and the other one for liquid medium conditioning. After 21 days of culture, biomass and scopolamine production were evaluated, resulting in the extraction of 9.5 mg of scopolamine/g of dry roots. This value was higher than the one obtained in a previous culture in Erlenmeyer flasks (6mg/g of dry roots) and higher than the scopolamine extracted from hairy roots cultures using a bioreactor reported in other studies. Regarding biomass, the growth index (GI) was 1.58, similar to the GI obtained with cultures in Erlenmeyer flasks (GI 1.4), which suggests that scaling up the process did not affect biomass productionnegatively.
Declaration of originality and transfer author's rights
The authors declare:
- The published data and reference materials have been duly identified with their respective credits and have been included in the bibliographic notes and citations that have been so identified and that should it be required, I have all releases and permissions from any copyrighted material.
- All material presented is free from any copyright and that I accept full legal responsibility for any legal claims relating to copyrighted intellectual property, fully exonerating from responsibility the Revista de la Academia Colombiana de Ciencias Exactas, Físicas y Naturales.
- This work is unpublished and will not be sent to any other journal while waiting for the editorial decision of this journal. I declare that there is no conflict of interest in this manuscript.
- In case of publication of this article, all author´s rights are transferred to the Revista de la Academia Colombiana de Ciencias Exactas, Físicas y Naturales, and so cannot be reproduced in any form without the express permission of it.
- By means of this document, if the article is accepted for publication by the Revista de la Academia Colombiana de Ciencias Exactas, Físicas y Naturales, the Revista assumes the right to edit and publish the articles in national and international indices or data bases for academic and scientific use in paper, electronic, CD-ROM, internet form either of the complete text or any other known form known or to be known and non-commercial, respecting the rights of the authors.
Transfer of author rights
In case the article is approved for publication, the main author in representation of himself and his co-authors or the main author and his co-authors must cede the author rights of the corresponding article to the Revista de la Academia Colombiana de Ciencias Exactas, Físicas y Naturales, except in the following cases:
The authors and co-authors will retain the right to revise, adapt, prepare derived works, oral presentations, and distribution to some colleagues of reprints of their own published work, if the corresponding credit is given to the Revista de la Academia Colombiana de Ciencias Exactas, Físicas y Naturales. It is also permissible to publish the title of the work, summary, tables, and figures of the work in the corresponding web sites of the authors or their employers, also giving credit to the Revista.
If the work has been realized under contract, the author’s employer has the right to revise, adapt, prepare derivative works, reproduce, or distribute in hard copy the published work, in a secure manner and for the exclusive use of his employees.
If the Revista de la Academia Colombiana de Ciencias Exactas, Físicas y Naturales were approached for permission by a third party for using, printing, or publishing specifically articles already published, the Revista must obtain the express permission of the author and co-authors of the work or of the employer except for use in classrooms, libraries, or reprinted in a collective work. The Revista de la Academia Colombiana de Ciencias Exactas, Físicas y Naturales reserves the possible use in its front cover of figures submitted with the manuscripts.
No other right, other than the author’s right, can be claimed by the Revista de la Academia Colombiana de Ciencias Exactas, Físicas y Naturales.