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We evaluated the amylolytic potential of microorganisms isolated from substrates related to rice crops cultured on starch extracted by the alkaline method from third category Índica and Fedearroz 60 rice varieties. The starch was subjected to direct fermentation with microorganisms isolated from the substrates; we evaluated the effect of the type of microorganism and the fermentation time (24, 48 and 72 h) in 50 ml reactors supplemented with 20 g/l of starch, and we measured total sugars, reducing sugars and direct amylolytic activity. Among the microorganisms recovered from the substrates, Aspergillus spp., Rhizopus oryzae, Penicillium spp. and Candida krusei were especially efficient given their growth rate in the starch-supplemented medium, and their differential amylolytic activity. The most efficient organism was Aspergillus spp., releasing 2.85 g/l of reducing sugar at 72 h in starch from the Fedearroz 60 rice variety, and the least efficient was C. krusei, with 0.300 g/l of reducing sugars during the same fermentation time, apparently regardless of the variety. This Índicates a significant amylolytic potential of native microorganisms associated with rice crops and their significant effect on the structure and chemical composition of the starch varieties under study. © 2015. Acad. Colomb. Cienc. Ex. Fis. Nat.References
Alva, S., Anupama, J., Savla, J., Chiu, Y., Vyshali, P., Shruti, M., Ruchi, K. (2007). Production and characterization of fungal amylase enzyme isolated from Aspergillus sp. JGI 12 in solid state culture. Afr.J. Biotechnol. 6 (5): 576-581.
Binod, P., Sindhu, R., Singhania, R. R., Vikram, S., Devi, L., Nagalakshmi, S., Pandey, A. (2010). Bioethanol production from rice straw: An overview. Bioresource Technol. 101(13): 4767-4774. doi: 10.1016/j.biortech.2009.10.079
Camargo, J. V. (2005). Diseño, construcción y puesta a punto de un prototipo de quemador para la combustión continua y eficiente de la cascarilla de arroz. El Hombre y la Máquina. 25: 128-135.
Dane-Fedearroz. (2015). Encuesta nacional de arroz mecanizado, I semestre de 2013. Dane-Fedearroz. Recuperado el 14 de noviembre, 2015, de http://www.dane.gov.co/files/investigaciones/boletines/arroz/bol_arroz_Isem15.pdf.
de Castro, A., de Andréa, T., Carvalho, D., Teixeira, M., dos Reis Castilho, L., Freire, D. (2011). Valorization of residual agroindustrial cakes by fungal production of multienzyme complexes and their use in cold hydrolysis of raw starch. Waste. Biomass. Valorization. 2 (3): 291-302. doi: 10.1007/s12649-011-9075-5
Espinal, C., Martínez, H., Ortíz, L. (2005). La cadena del arroz en Colombia, una mirada global de su estructura y dinámica, 1991-2005. Ministerio de Agricultura y Desarrollo Rural Observatorio Agrocadenas Colombia, 38. FAO. (2004). El arroz y la nutrición humana. Recuperado el 20 de marzo, 2014, de http://www.fao.org/rice2004/es/f-sheet/hoja3.pdf.
Gilman, I. (1963). Manual de los hongos del suelo (Primera edición). México DF: Compañía Editorial Continental.
Goyal, N., Gupta, J. K., Soni, S. K. (2005). A novel raw starch digesting thermostable α-amylase from Bacillus sp. I-3 and its use in the direct hydrolysis of raw potato starch. Enzyme. Microb. Tech. 37 (7): 723-734. doi: 10.1016/j.enzmictec.2005.04.017
Karimi, K., Emtiazi, G., Taherzadeh, M. J. (2006). Ethanol production from dilute-acid pretreated rice straw by simultaneous saccharification and fermentation with Mucor indicus, Rhizopus oryzae, and Saccharomyces cerevisiae. Enzyme. Microb. Tech. 40 (1): 138-144. doi: 10.1016/j.enzmictec.2005.10.046
Kim, H.-N., Sandhu, K. S., Lee, J. H., Lim, H. S., Lim, S.-T. (2010). Characterisation of 2-octen-1-ylsuccinylated waxy rice amylodextrins prepared by dry-heating. Food. Chem. 119 (3), 1189-1194. doi: 10.1016/j.foodchem.2009.08.036
Ko, J. K., Bak, J. S., Jung, M. W., Lee, H. J., Choi, I.-G., Kim, T. H., Kim, K. H. (2009). Ethanol production from rice straw using optimized aqueous-ammonia soaking pretreatment and simultaneous saccharification and fermentation processes. Bioresource. Tech.100 (19): 4374-4380. doi: 10.1016/j.biortech.2009.04.026
Miller, G. L. (1959). Use of Dinitrosalicylic Acid Reagent for Determination of Reducing Sugar. Anal. Chem. 31 (3): 426-428. doi: 10.1021/ac60147a030
Naguleswaran, S., Li, J., Vasanthan, T., Bressler, D., Hoover, R. (2012). Amylolysis of large and small granules of native triticale, wheat and corn starches using a mixture of α-amylase and glucoamylase. Carbohyd. Polym:88 (3): 864-874
Prasad, S., Singh, A., Joshi, H. C. (2007). Ethanol as an alternative fuel from agricultural, industrial and urban residues. Resour. Conserv. Recy. 50 (1): 1-39. doi: 10.1016/j.resconrec.2006.05.007
Roy, I., & Gupta, M. N. (2004). Hydrolysis of starch by a mixture of glucoamylase and pullulanase entrapped individually in calcium alginate beads. Enzyme. Microb. Tech. 34 (1): 26-32. doi: 10.1016/j.enzmictec.2003.07.001
Sarmiento, V., Vargas, D., Pedroza, A., Matiz, A., Piñales, R. P. (2003). Producción de α-Amilasa con celulas libres e inmovilizadas de Thermus sp. Revista MVZ Córdoba. 8(2): 310-317.
Sivaramakrishnan, S., Gangadharan, D., Nampoothiri, K. M., Soccol, C. R., Pandey, A. (2006). α-Amylases from microbial sources–an overview on recent developments. Food. Technol. Biotechnol.44 (2): 173-184.
Sodhi, H. K., Sharma, K., Gupta, J. K., Soni, S. K. (2005). Production of a thermostable α-amylase from Bacillus sp. PS-7 by solid state fermentation and its synergistic use in the hydrolysis of malt starch for alcohol production. Process. Biochem. 40 (2): 525-534. doi: 10.1016/j.procbio. 2003.10.008
Tahir, R., Ellis, P. R., Butterworth, P. J. (2010). The relation of physical properties of native starch granules to the kinetics of amylolysis catalysed by porcine pancreatic α-amylase. Carbohyd. Polym. 81 (1): 57-62. doi: 10.1016/j.carbpol.2010.01.055
Tester, R. F., Qi, X., Karkalas, J. (2006). Hydrolysis of native starches with amylases. Animal. Feed. Sci. Tech. 130 (1-2): 39-54. doi: 10.1016/j.anifeedsci.2006.01.016
Vanegas, I., Yepes, M., Duque, M. (2003). Búsqueda de cepas colombianas de levaduras productoras de xilitol. Facultad de Ciencias Agropecuarias. 28 (2): 27-32.
Vanegas, I. A., Yepes, M. d. S., Ruiz, O. S. (2004). Producción de xilitol a partir de levaduras nativas colombianas. Rev. Colomb. Biotecnol. 6 (2): 31-36.
Villalba, P., Bula, A., Juan, H. S., Ávila, A. (2008). Yucca (Manihot esculenta Crantz) starch polysaccharide dex-trination through biological procedures. Interciencia. 33(4): 314-316.
Wani, A. A., Singh, P., Shah, M. A., Schweiggert-Weisz, U., Gul, K., Wani, I. A. (2012). Rice starch diversity: Effects on structural, morphological, thermal, and physicochemical properties — A review. Compr. Rev. Food. Sci. F.11 (5): 417-436. doi: 10.1111/j.1541-4337.2012.00193.x
Zamora, R. J. V., Perea, E. M., Murillo, W. (2013). Caracterización de almidón extraído de arroz de tercera de las variedades Índica y Fedearroz 60.Tumbaga. 2 (8): 1.
Zhou, Y., Hoover, R., Liu, Q. (2004). Relationship between α-amylase degradation and the structure and physico-chemical properties of legume starches. Carbohyd. Polym.57 (3): 299-317
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