COMPARATIVE STUDY BY CAPILLARY ELECTROPHORESIS AND HIGH PERFORMANCE LIQUID CHROMATOGRAPHY OF CATECHINES EXTRACTED FROM FIVE VARIETIES OF COLOMBIAN CACAO
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Cala, M., Vásquez, Ángela, García, A., René Martínez, J., & Stashenko, E. (2023). COMPARATIVE STUDY BY CAPILLARY ELECTROPHORESIS AND HIGH PERFORMANCE LIQUID CHROMATOGRAPHY OF CATECHINES EXTRACTED FROM FIVE VARIETIES OF COLOMBIAN CACAO. Revista De La Academia Colombiana De Ciencias Exactas, Físicas Y Naturales, 35(136), 371–380. https://doi.org/10.18257/raccefyn.35(136).2011.2519

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Abstract

Analytical methodologies based on capillary electrophoresis (CE) and high-performance liquid chromatography (HPLC) were implemented for the separation and quantitation of (±)-catechine, (-)-epicatechine, (-)-epigallocatechine, (-)-epicatechine gallate, and (-)-epigallocatechine gallate in 5 samples of 3 cacao varieties grown in Colombia. HPLC was found to be more reproducible than CE, which turned out to be faster, more economical, and less contaminant. (±)-Catechine (0,07-0,79 mg/g cacao) and (-)-epicatechine (1,99-9,66 mg/g cacao) were determined in aqueous extracts of the 3 cacao varieties.

https://doi.org/10.18257/raccefyn.35(136).2011.2519

Keywords

catechines | CE | HPLC | Theobroma cacao
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References

Auger, C.; Al-Awwadi, N.; Bornet, A.; Rouanet, J.; Gase, F.; Cros G.; Teissedre P. 2004. Catechins and procyanidins in Mediterranean diets. Food Res Inter 37:233-245.

Bowser, M.; Frost, N.; Jing, M. 2010. Capillary electrophoresis. Anal Chem 82:4682-4698.

Castro, M.; López, J.; Gonzáles, M.; Barral, L. 2011. Development, validation and application of micellar capillary chromatography method for routine analysis of catechins, quercetin and thymol in natural samples. Microchem J 99:461-469.

Calderón, A.; Wright, B.; Hurts, J.; Breemen, R. 2009. Screening antioxidants using LC-MS: Case study with cocoa. J Agric Food Chem 57:5693-5699.

Dalluge, J.; Nelson, B. 2000. Determination of tea catechins. J Chromatogr A 880:411-424.

Folch-Cano, C.; Jullian, C.; Speisky, H.; Olea-Azar, C. 2010. Antioxidant activity of inclusion complexes of tea catechins with â-cyclodextrins by ORAC assays. Food Res Int 43:2039-2044.

Her, M.; Koel, M. 2003. Separation of polyphenolic compounds extracted from plant matrices using capillary electrophoresis. J Chromatogr A 990:225-230.

Kartsova L.; Ganzha O. 2006. Electrophoretic separation of tea flavanoids in the modes of capillary zone electrophoresis and micellar electrokinetic chromatography, Russ J Appl Chem 79:1110-1114.

Niemenak, N.; Rohsius, C.; Elwers, S.; Ndoumou D. O.; Lieberei R. 2006. Comparative study of different cocoa (Theobroma cacao L.) clones in terms of their phenolics and anthocyanins contents. J Food Composition Anal 19:612-619.

Nishitani, E.; Sagesaka Y. 2004. Simultaneous determination of catechins, caffeine and other phenolic compounds in tea using new HPLC method. J Food Comp Anal 17:675-685.

Putte, L. B.; Hollman, P. 2000. Catechin contents of foods commonly consumed in the Netherlands. 2. Tea, Wine, Fruit Juices, and Chocolate Milk. J Agric Food Chem 48:1752-1757.

Rice-Evans, C. 1996. Structure-antioxidant activity relationships of flavonoids and phenolic acids. Free Radical Biol Med 20(7):933-956.

Tsao, R.; Deng, Z. 2004. Separation procedures for naturally occurring antioxidant phytochemicals. J Chromatogr B 812:85-99.

Valls, J.; Millán, S.; Martí, M.; Borràs, E.; Arola, L. 2009. Advanced separation methods of food anthocyanins, isoflavones, and flavonols. J Chromatogr A 1216(43):7143-7172.

Vanhoenacker, G.; Villiers, A.; Lazou, K.; De Keukeleire D.; Sandra P. 2001. Comparison of high-performance liquid chromatography-mass spectroscopy and capillary electrophoresis-mass spectroscopy for the analysis of phenolic compounds in diethyl ether extracts of red wines. J Chromatogr A 54:309-315.

Yilmaz, Y. 2006. Novel uses of catechins in foods. Trends in Food Sci & Tech 17:64-71.

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