COMPARATIVE STUDY BY CAPILLARY ELECTROPHORESIS AND HIGH PERFORMANCE LIQUID CHROMATOGRAPHY OF CATECHINES EXTRACTED FROM FIVE VARIETIES OF COLOMBIAN CACAO
Vol. 35 No. 136 (2011), Chemical Sciences
Vol. 35 No. 136 (2011)

COMPARATIVE STUDY BY CAPILLARY ELECTROPHORESIS AND HIGH PERFORMANCE LIQUID CHROMATOGRAPHY OF CATECHINES EXTRACTED FROM FIVE VARIETIES OF COLOMBIAN CACAO

Chemical Sciences
https://doi.org/10.18257/raccefyn.35(136).2011.2519
Published 2023-12-22
Mónica Cala
Centro de Cromatografía y Espectrometría de Masas, CIBIMOL, Centro de Investigación de Excelencia CENIVAM, Universidad Industrial de Santander, Carrera 27, Calle 9, Bucaramanga, Colombia.
Ángela Vásquez
Centro de Cromatografía y Espectrometría de Masas, CIBIMOL, Centro de Investigación de Excelencia CENIVAM, Universidad Industrial de Santander, Carrera 27, Calle 9, Bucaramanga, Colombia.
Alejandro García
Centro de Cromatografía y Espectrometría de Masas, CIBIMOL, Centro de Investigación de Excelencia CENIVAM, Universidad Industrial de Santander, Carrera 27, Calle 9, Bucaramanga, Colombia.
Jairo René Martínez
Centro de Cromatografía y Espectrometría de Masas, CIBIMOL, Centro de Investigación de Excelencia CENIVAM, Universidad Industrial de Santander, Carrera 27, Calle 9, Bucaramanga, Colombia.
Elena Stashenko
Centro de Cromatografía y Espectrometría de Masas, CIBIMOL, Centro de Investigación de Excelencia CENIVAM, Universidad Industrial de Santander, Carrera 27, Calle 9, Bucaramanga, Colombia.
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How to Cite

Cala, M., Vásquez, Ángela, García, A., René Martínez, J., & Stashenko, E. (2023). COMPARATIVE STUDY BY CAPILLARY ELECTROPHORESIS AND HIGH PERFORMANCE LIQUID CHROMATOGRAPHY OF CATECHINES EXTRACTED FROM FIVE VARIETIES OF COLOMBIAN CACAO. Revista De La Academia Colombiana De Ciencias Exactas, Físicas Y Naturales, 35(136), 371–380. https://doi.org/10.18257/raccefyn.35(136).2011.2519
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Abstract

Analytical methodologies based on capillary electrophoresis (CE) and high-performance liquid chromatography (HPLC) were implemented for the separation and quantitation of (±)-catechine, (-)-epicatechine, (-)-epigallocatechine, (-)-epicatechine gallate, and (-)-epigallocatechine gallate in 5 samples of 3 cacao varieties grown in Colombia. HPLC was found to be more reproducible than CE, which turned out to be faster, more economical, and less contaminant. (±)-Catechine (0,07-0,79 mg/g cacao) and (-)-epicatechine (1,99-9,66 mg/g cacao) were determined in aqueous extracts of the 3 cacao varieties.

https://doi.org/10.18257/raccefyn.35(136).2011.2519

Keywords

catechines | CE | HPLC | Theobroma cacao
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