How to Cite
Downloads
Métricas Alternativas
Dimensions
Abstract
Digital lensless holographic microscopy (DLHM) as an imaging architecture with micrometer spatial resolution and operating with multispectral sources is shown. The principles and recent advances in the realm of this technology of microscopy are presented from the point of view of the contributions of our group. DLHM operating a full color and with femto-second lasers are the advances highlighted in this work. The effects of the spatial and the temporal coherence of the light source on the performance of the DLHM are analyzed by comparing the reconstructed images of the microscope by using a LED and a 12 fs mode-locked Ti:Sa laser. A section of the head of a Drosophila melanogaster fly is utilized as a sample with complex internal structure. © 2015. Acad. Colomb. Cienc. Ex. Fis. Nat. All rights reserved.References
Barton, J. J. (1988). Photoelectron Holography. Physical Review Letters, 61 (12). American Physical Society, 1356-59.
Bodian, D. (1936). A New Method for Staining Nerve Fibers and Nerve Endings in Mounted Paraffin Sections. The Anatomical Record, 65 (1). Wiley Subscription Services, Inc., A Wiley Company, 89-97.
Brunel, M., Shen, H., Coetmellec, S., Lebrun, D., and Ait Ameur, H. (2012). Femtosecond Digital in-Line Holography with the Fractional Fourier Transform: Application to Phase-Contrast Metrology. Applied Physics B, 106 (3). Springer-Verlag, 583-91.
Cuche, E., Bevilacqua, H., and Depeursinge, C. (1999). Digital Holography for Quantitative Phase-Contrast Imaging. Opt. Lett., 24 (5). OSA, 291-93.
Fink, H-W., Schmid, H., Kreuzer, H., and Wierzbicki, A. (1991). Atomic Resolution in Lensless Low-Energy Electron Holography. Physical Review Letters, 67 (12): 1543-46.Gabor, D. (1948). A New Microscopic Principle. Nature, 161:777-78.
Gabor, D. (1949). Microscopy by Reconstructed Wave-Fronts. Proc. R. Soc. London A, 197: 454.
Gabor, D. (1951). Microscopy by Reconstructed Wave Fronts: II. Proc. Phys. Soc. London B, 64: 449.
Garcia-Sucerquia, J., Trujillo, C., and Restrepo, J. (2014). Microscopio Holográfico Digital Sin Lentes (MHDSL) y Método Para Visualizar Muestras. Colombia: SIC (Colombia).
Garcia-Sucerquia, J. (2012). Color Lensless Digital Holographic Microscopy with Micrometer Resolution. Optics Letters, 37 (10): 1724-26.
Garcia-Sucerquia, J. (2013). Noise Reduction in Digital Lensless Holographic Microscopy by Engineering the Light from a Light-Emitting Diode. Applied Optics, 52 (1): A232–39.
Garcia-Sucerquia, J., Herrera-Ramírez, J., Castaneda, R.(2006). Incoherent Recovering of the Spatial Resolution in Digital Holography. Optics Communications, 260 (1): 62-67.
Garcia-Sucerquia, J., Xu, W., Jericho, S., Klages, P., Jericho, M., and Kreuzer, H. (2006). Digital in-Line Holographic Microscopy. Appl. Opt., 45 (5). OSA, 836-50.
Goodman, J W, and Lawrence, L. (1967). Digital image formation from electronically detected holograms. Applied Physics Letters, 11 (3).
Goodman, J W. (2005). Introduction to Fourier Optics. Greenwood Village: Roberst & Company Publishers.Gu, M. (2000). Advanced Optical Imaging Theory. Springer Series in Optical Sciences,. Vol. 75. Springer.
Jericho, M H, and Kreuzer, H. (2011). Point Source Digital In-Line Holographic Microscopy. In Coherent Light Microscopy, edited by P Ferraro, A Wax, and Z Zalevvsky, 3–30. Springer-Verlag Berlin Heidelberg.Kreis, T. (2002). Frequency Analysis of Digital Holography. Optical Engineering, 41 (4): 771-78.
Kreuzer, H. (2002). “Holographic microscope and method of hologram reconstruction,” US,641140.
Kreuzer, H J, Fink, H., Schmid, H., and Bonev, S. (1995). Holography of Holes, with Electrons and Photons. Journal of Microscopy, 178 (3), 191-97.
Leith, N., and Upatnieks, J. (1964). Wavefront Reconstruction with Diffused Illumination and Three-Dimensional Objects. Journal of the Optical Society of America, 54 (11). OSA, 1295–1301.
Mendoza-Yero, O., Calabuig, A., Tajahuerce, E., Lancis, J., Andrés, P., and Garcia-Sucerquia, J. (2013). Femtosecond Digital Lensless Holographic Microscopy to Image Biological Samples. Optics Letters, 38 (17). OSA, 3205-7.
Mendoza-Yero, O., Tajahuerce, E., Lancis, J., and Garcia-Sucerquia, J. (2013). Diffractive Digital Lensless Holo-graphic Microscopy with Fine Spectral Tuning. Optics Letters, 38 (12): 2107-9.
Petruck, P, Riesenberg, R., and Kowarschik, R. (2012). Optimized Coherence Parameters for High-Resolution Holographic Microscopy. Applied Physics B, 106 (2). Springer-Verlag, 339-48.
Picart, P., and Leval, J. (2008). General Theoretical Formulation of Image Formation in Digital Fresnel Holography: Erratum. J. Opt. Soc. Am. A, 26 (2). OSA, 244.
Repetto, L, Piano, E., and Pontiggia, C. (2004). Lensless Digital Holographic Microscope with Light-Emitting Diode Illumination. Opt. Lett., 29 (10). OSA, 1132-34.
Rosenhahn, A., Staier, F., Nisius, T., Schäfer, D., Barth, R., Christophis, C., Stadler, M. et al. (2009). Digital In-Line Holography with Femtosecond VUV Radiation Provided by the Free-Electron Laser FLASH. Opt. Express, 17 (10). OSA, 8220-28.
Schnars, U. (1994). Direct Phase Determination in Hologram Interferometry with Use of Digitally Recorded Holograms. J. Opt. Soc. Am. A, 11 (7). OSA, 2011-15.
Trujillo, C, and Garcia-Sucerquia, J. (2013). Accelerated Numerical Processing of Electronically Recorded Holograms With Reduced Speckle Noise. Image Processing, IEEE Transactions on, 22 (9): 3528-37.
Witte, S., Plauska, A., Ridder,M., Berge, Huibert D Mansvelder, H., and Groot, M. (2012). Short-Coherence off-Axis Holographic Phase Microscopy of Live Cell Dynamics. Biomed. Opt. Express, 3 (9). OSA, 2184–89.
Declaration of originality and transfer author's rights
The authors declare:
- The published data and reference materials have been duly identified with their respective credits and have been included in the bibliographic notes and citations that have been so identified and that should it be required, I have all releases and permissions from any copyrighted material.
- All material presented is free from any copyright and that I accept full legal responsibility for any legal claims relating to copyrighted intellectual property, fully exonerating from responsibility the Revista de la Academia Colombiana de Ciencias Exactas, Físicas y Naturales.
- This work is unpublished and will not be sent to any other journal while waiting for the editorial decision of this journal. I declare that there is no conflict of interest in this manuscript.
- In case of publication of this article, all author´s rights are transferred to the Revista de la Academia Colombiana de Ciencias Exactas, Físicas y Naturales, and so cannot be reproduced in any form without the express permission of it.
- By means of this document, if the article is accepted for publication by the Revista de la Academia Colombiana de Ciencias Exactas, Físicas y Naturales, the Revista assumes the right to edit and publish the articles in national and international indices or data bases for academic and scientific use in paper, electronic, CD-ROM, internet form either of the complete text or any other known form known or to be known and non-commercial, respecting the rights of the authors.
Transfer of author rights
In case the article is approved for publication, the main author in representation of himself and his co-authors or the main author and his co-authors must cede the author rights of the corresponding article to the Revista de la Academia Colombiana de Ciencias Exactas, Físicas y Naturales, except in the following cases:
The authors and co-authors will retain the right to revise, adapt, prepare derived works, oral presentations, and distribution to some colleagues of reprints of their own published work, if the corresponding credit is given to the Revista de la Academia Colombiana de Ciencias Exactas, Físicas y Naturales. It is also permissible to publish the title of the work, summary, tables, and figures of the work in the corresponding web sites of the authors or their employers, also giving credit to the Revista.
If the work has been realized under contract, the author’s employer has the right to revise, adapt, prepare derivative works, reproduce, or distribute in hard copy the published work, in a secure manner and for the exclusive use of his employees.
If the Revista de la Academia Colombiana de Ciencias Exactas, Físicas y Naturales were approached for permission by a third party for using, printing, or publishing specifically articles already published, the Revista must obtain the express permission of the author and co-authors of the work or of the employer except for use in classrooms, libraries, or reprinted in a collective work. The Revista de la Academia Colombiana de Ciencias Exactas, Físicas y Naturales reserves the possible use in its front cover of figures submitted with the manuscripts.
No other right, other than the author’s right, can be claimed by the Revista de la Academia Colombiana de Ciencias Exactas, Físicas y Naturales.