How to Cite
Downloads
Métricas Alternativas
Dimensions
Abstract
The complete nucleotide sequence of the genomic RNA of the NL4 strain of Bean common mosaic virus (BCMV-NL4) was determined. The viral genome is 10037 nucleotides in length, excluding the 3’ terminal poly (A) tail, and contains a single open reading frame (ORF) of 9666 nucleotides encoding a polyprotein of 3222 amino acids. The ORF is flanked by 5’ and 3’ untranslated regions (UTRs) of 133 and 235 nucleotides, respectively. Comparative analyses of the predicted BCMV-NL4 polyprotein with other species of the genus Potyvirus revealed nine cleavage sites resulting in ten functional proteins. Nucleotide and amino acid sequence identities indicated a close relationship between BCMV-NL4 and BCMV-NL1. Blast comparisons using coat protein (CP) and 3’UTR sequences showed 100% identity between BCMV-NL4 and the Mexican variant (US6) of this strain.
Keywords
References
Adams MJ, Antoniw JF, Fauquet CM. 2005. Molecular criteria for genus and species discrimination within the family Potyviridae. Arch Virol 150: 459-479.
Atreya CD. 1992. Application of genome sequence information in potyvirus taxonomy: an overview. Arch Virol Suppl 5: 17-23.
Berger PH, Wyatt SD, Shiel PJ, Silbernagel MJ, Druffel MJ, Mink GI. 1997. Phylogenetic analysis of the Potyviridae with emphasis on legume-infecting potyviruses. Arch Virol 142:1979-1999.
Chen J, Adams MJ, Zheng HY, Chen JP. 2003. Sequence analysis demonstrates that Onion yellow dwarf virus isolates from China contain a P3 region much larger than other potyviruses. Arch Virol 148: 1165-1173.
CIAT-CABI. 1991. Common Beans: Research for Crop Improvement. (A.v. Schoonhoven and O. Voysest, Eds.). Redwood Press, Melksham, U.K. 980 p.
Domier LL, Shaw JG, Rhoads RE. 1987. Potyviral proteins share amino acid sequence homology with picorna-, como-, and caulimoviral proteins. Virology 158: 20-27.
Dougherty WG, Carrington JC. 1988. Expression and function of potyviral gene products. Annu Rev Phytopathol 26 : 123-143.
Dougherty WG, Parks TD, Cary SM, Bazan JF, Fletterrick RJ. 1989. Characterisation of the catalytic residues of the tobacco etch virus 49-kDa proteinase. Virology 172: 302–310.
Dreher TW, Miller WA. 2006. Translational control of positive strand RNA plant viruses. Virology 344: 185-197.
Drijfhout E. 1978. Genetic interactions between Phaseolus vulgaris and bean common mosaic virus with implications for strain identification and breeding for resistance. Centre for Agricultural Publishing and Documentation. Wageningen. 98 p.
Frenkel MJ, Ward CW, Shukla DD. 1989. The use of 3 noncoding nucleotide sequences in the taxonomy of potyviruses: Application to watermelon mosaic virus 2 and soybean mosaic virus-N. J Gen Virol 70: 2775-2783.
Gal-On A. 2000. A point mutation in the FRNK motif of the potyvirus helper component-protease gene alters symptoms expression in cucurbits and elicits protection against the severe homologous virus. Phytopathology 90: 467-473.
Kong P, Steinbiss HH. 1998. Complete nucleotide sequence and analysis of the putative polyprotein of maize dwarf mosaic virus genomic RNA (Bulgarian isolate). Arch Virol 1 4 3 (9):1791-1799.
Laín S, Riechmann JL, García JA. 1989. The complete nucleotide sequence of plum pox potyvirus RNA. Virus Res 13 (2):157-172.
Levy S, Avni D, Hariharan N, Perry RP, Meyuhas O. 1991. Oligopyrimidine tract at the 5’ end of mammalian ribosomal protein mRNAs is required for their translational control. Proc Natl Acad Sci USA 88: 3319-3323.
Mlotshwa S, Verver J, Sithole-Niang I, Van Kampen T, Van Kammen A, Wellink J. 2002. The genomic sequence of Cowpea aphid-borne mosaic virus and its similarities with other potyviruses. Arch Virol 147: 1043-1052.
Morales FJ. 1979. Purification and serology of bean common mosaic virus. Turrialba 29: 320-323.
Morales FJ. 1980. El mosaico común del fríjol. Metodología de investigación y técnicas de control. CIAT. 22 p.
Plisson C, Drucker M, Blanc S, German-Retana S, Le Gall O, Thomas D, Bron P. 2003. Structural characterization of HC-Pro, a plant virus multifunctional protein. J Biol Chem 2 7 8: 23753-23761.
Riechmann JL, Lain S, Garcia JA. 1992. Highlights and prospects of potyvirus molecular biology. J Gen Virol 73: 1-16.
Robaglia C, Durand-Tardif M, Tronchet M, Boudazin G, Astier-Manifacier S, Casse-Delbart F. 1 9 8 9. Nucleotide sequence of potato virus Y (N Strain) genomic RNA. J Gen Virol 70: 935-947.
Shukla DD, Frenkel MJ, McKern NM, Ward CW, Jilka J, Tosic M, Ford RE. 1992. Present status of the sugarcane mosaic subgroup of potyviruses. Arch Virol Suppl 5: 363-373
Silbernagel MJ. 1969. Mexican strain of bean common mosaic virus. Phytopathology 59: 1809-1812.
Spetz C, Valkonen JPT. 2003. Genomic sequence of Wild potato mosaic virus as compared to the genomes of other potyviruses. Arch Virol 148: 373-380.
Thompson JD, Higgins DG, Gibson TJ. 1994. CLUSTAL W: Improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22 : 4673-4680.
Turpen T. 1989. Molecular cloning of a potato virus Y genome: nucleotide sequence homology in non-coding regions of potyviruses. J Gen Virol 70: 1951-1960.
Urcuqui-Inchima S, Haenni AL, Bernardi F. 2001. Potyvirus proteins: a wealth of functions. Virus Res 74: 157-175.
Zheng H, Chen J, Chen J, Adams MJ, Hou M. 2002. Bean common mosaic virus isolates causing different symptoms in asparagus bean in China differ greatly in the 5’-parts of their genomes. Arch Virol 147: 1257-1262.
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
Copyright (c) 2023 Revista de la Academia Colombiana de Ciencias Exactas, Físicas y Naturales