EFECTO DE LA POSICIÓN DEL ÁCIDO ASPÁRTICO EN LA SOLUBILIDAD Y EN LA ADSORCIÓN SOBRE HIDRÓXIDO DE ALUMINIO DE UNA SERIE DE PÉPTIDOS ANÁLOGOS

Mary  Trujillo; Luis A.  Oviedo; Fanny  Guzmán; Julio C.  Calvo

doi:10.18257/raccefyn.29(111).2005.2162

Vol. 29 No. 111 (2005), Chemical Sciences

Vol. 29 No. 111 (2005)

ASPARTIC ACID POSITION EFFECT IN SOLUBILITY AND ADSORPTION ONTO ALUMINIUM HYDROXIDE IN AN ANALOGUE PEPTIDE SERIES

Chemical Sciences

https://doi.org/10.18257/raccefyn.29(111).2005.2162

Published 2023-10-18

Mary Trujillo⁺⁻
Luis A. Oviedo⁺⁻
Fanny Guzmán ⁺⁻
Julio C. Calvo⁺⁻

Mary Trujillo

Fundación Instituto de Inmunología de Colombia, FIDIC, Bogotá, D.C., Colombia. Dpto. Química, Fac. Ciencias, Universidad Nacional de Colombia, Bogotá, D.C., Colombia

Luis A. Oviedo

Dpto. Química, Fac. Ciencias, Universidad Nacional de Colombia, Bogotá, D.C., Colombia.

Fanny Guzmán

Fundación Instituto de Inmunología de Colombia, FIDIC, Bogotá, D.C., Colombia.

Julio C. Calvo

Grupo Proteoma, Lab. Biología-Química, Fac. Ciencias y Educación, Universidad Distrital Fco. José de Caldas, Cra. 4 No. 26-B-54, Bogotá, D.C., Colombia

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How to Cite

Trujillo, M. ., Oviedo, . L. A. ., Guzmán , F. ., & Calvo, J. C. . (2023). ASPARTIC ACID POSITION EFFECT IN SOLUBILITY AND ADSORPTION ONTO ALUMINIUM HYDROXIDE IN AN ANALOGUE PEPTIDE SERIES. Revista De La Academia Colombiana De Ciencias Exactas, Físicas Y Naturales, 29(111), 283–288. https://doi.org/10.18257/raccefyn.29(111).2005.2162

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Abstract

Two analogue series of a peptide sequence from the 42 kD fragment of Plasmodium falciparum MSP-1 protein were synthesized by systematically replacing each one of the positions by aspartic acid or lysine to study the physical adsorption phenomenon. Such modifications showed a nonregular tendency regarding solubility and adsorption data. The aspartic acid best adsorption data was obtained by substituting aspartic acid in position 6, and the worst in position 5; however, no significant structural differences were found between these analogue peptides. Decreasing adsorption values in the lysine analogue series, indicate that adsorption into aluminium hydroxide is predominantly guided by electrostatic effects.

https://doi.org/10.18257/raccefyn.29(111).2005.2162

Keywords

Adsorption | Aluminium hydroxide | Solubility, | Peptide analogues | Aspartic acid

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References

Al-Shakhshir, R. H., Regnier, F. E., White, J. L., & S. L. Hem. 1994. Contribution of electrostatic and hydrophobic interactions to the adsorption of proteins by aluminium-containing adjuvants. Vaccine 12: 472-474.

Al-Shakhshir, R. H., Lee, A. L., White, J. L., & S. L. Hem. 1995, Interaction in model vaccines composed of mixtures of aluminium-containing adjuvants. J. Colloid Interface Sci. 169: 197-199

Al-Shakhshir, R. H., Regnier, F. E., White, J. L., & S. L. Hem. 1995b. Contribution of electrostatic and hydrofobic interactions to the adsorption of proteins by aluminum-containing adjuvants. Vaccine 13: 41-44.

Blackman, M. J. & A. A. Holder. 1992. Secondary processing of the Plasmodium falciparum merozoite surface protein by a calcium-dependent membrane-bound serine protease shedding of MSP as a noncovalently associated complex with other fragments of the MSP-1. Molec. And. Biochem. Parasitol., 49: 29-34

Brown, R. E., Jarvis, K. L. & K. J. Hyland, K. 1989. Protein measurement using Bicinchoninic acid: Elimination of interfering substances. Anal. Biochem. 180: 136-139

Callahan, P. M., Shorter, A. L, &. S. L. Hem. 1991. The importance of surface charge characteristics of aluminum-containing adjuvants. Vaccine 12:472-474.

Chang, M., White, J. L., Nail S. L. & S. L. Hem. 1997. Role of the electrostatic attractive force in the adsorption of proteins by aluminium hydroxide adjuvant. PDA J. Pharm. Sci. & Tech. 51(1): 25-29

Espejo, B. F., Cubillos, M., Salazar, L. M., Guzmán, F., Urquiza, M., Ocampo, M., Silva, Y., Rodríguez, R., Lyoi, E. & M. E. Patarroyo. 2001. Structure, Immugenicity, and Protectivity relationship for the 1585 malarial peptide and its substitution analogues. Angew. Chem. Int. Ed. 40: 4654-4657

Hem, S. L. & J. L. White. 1984. Characterization of aluminium hydroxide for use as an adjuvant in parenteral vaccines. J. Parenteral Sc. And Tech. 38(1): 1-10.

Hem, S. L. &. J. L. White. En: Powell M.F, Newman M.J., and Burdman J.R. editors. 1995. Vaccine design-the subunit and adjuvant approach. New York: Plenum Press, 249-75.

Houghten, R. 1985. General method for the rapid solid-phase synthesis of large number of peptides: specificity of antigen-antibody interaction at the level of individual amino acids. Proc. Natl. Acad. Sci., 82: 5131-5.

Martin, A., Swarbrick, J. & A. Camarata. 1983. A Physical Pharmacy, 3 rd edition, Lea & Febiger (Eds.), Philadelphia. Matheis, W., Zott, A. & M. Schwanig. 2002. The role of the adsorption process for production and control combined adsorbed bacines. Vaccine 20: 67-73.

Merrifield, R. B. 1963. Solid phase peptide synthesis I. The synthesis of a tetrapeptide. J. Am.Chem. Soc. 85: 2149-2154.

Rinella, J. V.jr., White, J. L., & S. L. Hem. 1995. Effect of anions on model aluminium adjuvant containing vaccines. J. Colloid Interface Sci. 172:121-130.

Seeber, S. J., White, J. L., & S. L. Hem. 1991a. Solubilization of aluminum-containing adjuvants by constituents of interstitial fluid. J. Parenteral Sci. Tech. 45: 156-159

Seeber, S. J., White, J. L., & S. L. Hem. 1991b. Predicting the adsorption of proteins by aluminum-containing adjuvants. Vaccine 9: 201-203.

Serna, C. J., White, J. L. & S. L. Hem. 1977. Anion-aluminium hydroxide gel interactions. Soil Sci. Soc. Am. J. 41: 1009-1013.

Serna, C. J., White, J. L., & S. L. Hem. 1978, Factors affecting homogeneous precipitation of aluminium hydroxide gel. J. Pharm. Sci. 67(8):1179-1181.

Shirodkar, S., Hutchinson, R. L., Perry, D. L., White, J. L., & S. L. Hem. 1990. Aluminum compounds used as adjuvants in vaccines, Pharm. Res. 2:1282-1288.

Smith, P. K., Krohn, R., Hermanson, A., Mallia, K., Gartner, F., Provenzano, M., Fujimoto, E., Goeke, N., Olson, B., & D. Klenk. 1985, Measurement of protein using Bicinchoninic acid. Anal. Biochem. 150: 76-85

Wiechelman, K., Braun, R. & J. Fitzpatrick. 1988. Investigation of the Bicinchoninic acid protein assay: identification of the groups responsible for color formation. Anal. Biochem. 175: 231-237.

World Health Organization (W.H.O.). 1976. Immunological adjuvants. World Health Organization Technical Report Series No. 595. Geneva: WHO, pp.6-8.

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