Keywords
Dioclea lehmanni
Erythrina costaricensis
mitogenesis
lymphocytes
Aotus l. griseimembra
Proechimys chrysaeolus
How to Cite
Societal impact
Abstract
Toe Erythrina costaricensis transforms the Proechimys chrysaeolus lymphocytes at a concentration of 0.65 lg/ml, the Aotus l. griseimembra lymphocytes at 2.38 lg/ml and human lymphocytes at 19.65 lg/ml. The three species differ in the concentrations required to reach maximal transfonnation; maximum stimulus is obtained with phytohaemaglutinin and favin. The Dioclea lehmanni P2 lectin stimulates only the Aotus l. griseimembra lymphocytes at a concentration of 1.34 lg/ml. Given the structural similarity of favin with the Erythrina lectins, the reported data are puzzling. Sorne hypothesis are laid down to explain the results.
References
Bryan, J.H.D. & R.L. Hybertson. 1972. The in vitro stimulation of lymphocytes from peripheral blood and lymph nodes of the laboratory mouse. Cytogenetics 11: 25-34.
Bueno M.L., M. Gómez-Laverde & A. Morales. 1989. Caracterización cariológica de Proechimys sp. (Rodentia: Echimyidae) de una colonia experimental. Biomédica 9: 13-22.
Gilbou-Garber, L & M. Mizrahi. 1981. A new mitogenic D-galactosephilic lectin isolated from seed of coral tree Erythrina corallodendron. Comparison with Glycine max (Soybean) and Pseudomonas aeruginosa lectins. Can. J. Biochem. 59: 315-320.
Giraldo, A., M.L. Bueno & E. Silva. 1986. Estudio citoge-nético de 288 Aotus Colombianos. Biomédica 6: 5-13.
Hershkovitz, P. 1983. Two new species of night monkeys, genus Aotus (Cebidae, Platyrrhini): A preliminary report on Aotus taxonomy. Am. J. Prim. 4: 209-243.
Iglesias, J. L., H. Lis & N. Sharon. 1982. Purification and properties of D-galactose/N-acetyl-D-galactosamine- specific lectin from Erythrina cristagalli. Eur. J. Biochem. 123: 247-253.
Kocourek, J. 1986. Historical Background. In: Liener, I.E., Sharon, N. and I.J. Goldstein (eds.) The Lectins: Properties, Functions, and Applications in Biology and Medicine: Academic Press, New York, pags 3-32.
Lis, H. & N. Sharon. 1986a. Aplicaciones de Lectins. In: Liener, I.E., Sharon, N. and I.J. Goldstein (eds.) The Lectins: Properties, Functions and Applications in Biology and Medicine: Academic Press, New York, pags 294-370.
Lis, H. & N. Sharon. 1986b. Biological Properties of Lect ins. In: Liener, I.E., Sharon, N. and I.J. Goldstein (eds.) The Lectins: Properties, Functions, and Applications in Biology and Medicine: Academic Press, New York, pags 266-293.
Lis, H., N. Sharon, J.F. Joubert, & N. Sharon. 1985. Isolation and properties of N-acetylactosamine-specific lectins from nine Erythrina species. Phytochemistry 24: 2803-2809.
Moorhead, P.S., P.C. Nowell, W.J. Mellman, D.M. Battips & D.A. Hungerford. 1960. Chromosome preparations of leukocytes cultured from human peripheral blood. Exp. Cell. Res. 20: 613-616.
Nanne, C.I. & F. Aragón. 1991. Aislamiento, purificación y caracterización de una lectina de la semilla del poró, Erythrina costaricensis (Leguminosae). Rev. Biol. Trop., 39: 15-21.
Nowell, P.C. 1960. Phytohemagglutinin: An initiator of mitosis in cultures of normal human leukocytes. Cancer Res. 20: 462-466.
Peña C., F. Villarraga & G. Pérez. 1988. A lectin from the seeds of Erythrina rubrinervia. Phytochemistry 27: 1045-1048. Pérez G. 1993a. Erythrina lectins-A review. Structural and physicochemical properties. Rev. Acad. Col. Ciencias 18: 545-553.
Pérez G. 1993b. Isolation and characterization of a lectin from the seeds of Erythrina costaricensis (en prensa).
Pérez, G., M. Hernández & E. Mora. 1990. Isolation and characterization of a lectin from the seeds of Dioclea lehmannii. Phytochemistry 29: 1745-1749.
Taylor, D.W. & W.A. Siddiqui. 1978. Comparative mitogen responses of lymphocytes from Colombian, Panamanian, and Peruvian owl monkeys. Lab. Ann. Sc. 28: 567-574.
Wilson M.F. & R.D. Pearson. 1984. Stage specific variations in lectin binding to Leishmania donovani. Infect. Immunol. 46: 128-134.
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