Keywords
How to Cite
Societal impact
Abstract
In this work a method was established for measuring the activity of PAL in crude extracts of coffee leaves. The product of the PAL reaction trans-cinnamic acid, was quantitated by high performance liquid chromatography, after its extraction with chloroform. The presence of the enzyme in leaves of resistant (Timor Hybrid and Colombia) and susceptible varieties (caturra) to Hemileia vastatrix Berk & Br., was studied. According to the results, there is no significant difference in the basal activity of PAL among rust resistant materials. In the varieties Colombia and Timor Hybrid; the values were 10,6 ± 3,0 and 13,18 ± pKat/mg protein, respectively. These resistant materials showed basal values of activity greater than those found for the Caturra variety, (3,57 ± 0,6 pKat/mg protein).
References
BRADFORD M., 1976. A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principie of protein binding. Anal. Biochem., 72: 248-254.
DUQUE A. & O. LOPEZ, 1987. Análisis de compuestos fenólicos presentes en explantes de hojas, callos y células en suspensión de café (Coffea arabica L.). Tesis de Grado, Departamento de Química y Farmacia. Universidad Nacional de Colombia, Bogotá, Colombia.
KOUKOL J. & E. CONN, 1961. The metabolism of aromatic compounds in higher plants. Purification and properties of the Phenylalanine deaminase of Hordeum vulgare. J. Biol. Chem., 236: 2692-2698.
MARTÍNEZ P. & P. RESTREPO, 1981. Detección y localización de la polifenoloxidasa y la L-fenilalanina amonio liasa en hojas de café caturra y efecto de un fungicida cúprico sobre la actividad de estas dos enzimas. Tesis de grado, Departamento de Química, Universidad Nacional de Colombia, Bogotá, Colombia.
MERCK INDEX, 1983. Tenth Ed. Merck and Co. Inc. Rahway N.Y.; USA.
MOERSCHBACHER B., KOGEL H.K., NOLL U. & REISENER H.J., 1986. An elicitor of the hypersensitive lignification response in wheat leaves isolated from the rust fungus Puccinia graminis f.sp. tritici. Partial purification and characterization. Z. Naturforsch 41, 830-838.
SIEVERS, S., 1983. The intracellular washing fluid of Coffea leaves as tool to study resistance mechanism against Hemileia vastatrix Berk & Br. In Simposio sobre ferrugens de cafeeiro. Oeiras Portugal 7-20-X-1983. Sumarios das comunicacões. Oeiras Centro de Investigação das Ferrugens do Cafeeiro. pp 24.
TOBAR M. & H. ZAMORA., 1982. Determinación de la L-fenilalanina amonio liasa en extractos de hojas de cuatro variedades de cafeto y en otros tejidos vegetales. Tesis de grado, Departamento de Química, Universidad Nacional de Colombia, Bogotá, Colombia.
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
Copyright (c) 2025 Revista de la Academia Colombiana de Ciencias Exactas, Físicas y Naturales