Archivos suplementarios
Palabras clave
respuesta SOS
ciprofloxacino
pinocembrina
inhibición
Lippia origanoides
Cómo citar
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Societal impact
Resumen
La resistencia antibiótica constituye un problema creciente debido al aumento de enfermedades asociadas con patógenos resistentes. La respuesta SOS bacteriana es una vía génica de respuesta al estrés cuyo papel principal es la reparación del ADN. Dicha respuesta puede activarse por el daño en el ADN causado por antibióticos y contribuye indirectamente a la aparición de resistencia a través de las polimerasas mutagénicas, la transferencia horizontal de genes y la promoción de la persistencia y la tolerancia bacteriana a los antibióticos. La inhibición de esta vía representa una estrategia prometedora para reducir la generación de variantes resistentes. En este contexto, nuestro objetivo fue evaluar las capacidades antibacterianas, moduladoras de antibióticos e inhibitorias de la respuesta SOS del extracto de Lippia origanoides (ELO) y la pinocembrina, utilizando la cepa Escherichia coli PQ37 y el ensayo SOS Chromotest. El antibiótico evaluado fue el ciprofloxacino, el cual demostró ser un potente inductor de la respuesta SOS en E. coli. En los tratamientos concomitantes con este antibiótico, la pinocembrina (0.097 mM) exhibió un efecto sinérgico sobre la actividad del antibiótico, reduciendo en cerca de cuatro veces su CMI. Además, la pinocembrina inhibió la respuesta SOS inducida por el ciprofloxacino de forma dependiente de la concentración, observándose una inhibición significativa a partir de 0.031 mM (r = - 0.87, p < 0.05). Nuestros resultados indican que la pinocembrina puede actuar como coadyuvante en la terapia antibiótica combinada, dado que potencia su actividad a la vez que inhibe la respuesta SOS bacteriana, un mecanismo que podría minimizar el riesgo de desarrollar resistencia a antibióticos.
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