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71 tumores humanos malignos y 20 benignos fueron estudiados en relación con su capacidad de utilizar oxígeno con sustratos endógenos o con glucosa, glutamina o una mezcla de las dos como sustratos. Se encontró que los tumores consumen oxígeno en forma muy similar a la de los tejidos peritumorales que fueron ablados en la misma operación quirúrgica. No se encontró diferencia en el consumo de glucosa o de glutamina por los tumores, pero se constató que la glutamina disminuía la captación de glucosa, mientras que la glucosa aumentaba la captación de glutamina. Se demostró que la marca radioactiva de la glucosa podía aparecer en CO2 , constatando que no hay una ruptura completa entre glicólisis y ciclo de ácidos tricarboxílicos en tumores humanos. Se propone que el aumento en lactato típico de los tumores puede ser debido a inhibición de los transportadores de equivalentes reducidos del citoplasma a la mitocondria acompañado de un descontrol de la enzima fosfofructocinasa.
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