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La actividad de las enzimas ligninolíticas, lignina peroxidasa (LiP), manganeso peroxidasa (MnP) y lacasa, en cultivos sumergidos de Phanerochaete chrysosporium, con cantidades limitadas de carbono y nitrógeno, se vio afectada por la adición de Mn+2• En los cultivos con O y 1,25 ppm de Mn+2, solo se detectó la LiP y su mayor actividad se presentó en los que contienen 1.25 ppm de Mn+2• En los cultivos con 40 ppm de Mn+2 se encontró actividad de LiP, de MnP y Jacasa. La presencia de las tres enzimas en el mismo cultivo no había sido reportada y es de gran importancia porque amplía las opciones que tiene el hongo y su maquinaria ligninolítica para actuar secuencialmente en aplicaciones biotecnológicas.
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