Abstract
Environmental scanning electron microscopy (ESEM) was performed in seedlings of Passiflora edulis f. flavicarpa inoculated with Fusarium solani f. sp. passiflorae (teleomorph: Haematonectria haematococca) causal agent of passionfruit collar rot. Inoculations were carried out every 24 h until the seventh day and from this day until the fifteenth the interval of inoculation was 72 h. The pathogen isolated in PDA media was placed on the surface of the collar through the modified test-tube screening methodology. Epidermis of collar, stem, and leaves and longitudinal sections of collar and stem were observed. After 24 h of inoculation, conidia and dense septate mycelium were observed over the epidermis of the stem and the collar, respectively. Hypertrophy and cell wall degradation of the vascular tissues were also found during this period. Five days after the inoculation macroconidia were formed from monophialides in the aerial mycelium on the stem. Ten days after inoculation, xylem and pith cells of the collar were colonized by hyphae, inclusions, and mature sporodoquia over the stem surface. Hyphae colonization started six days after inoculation on stomata and thirteen days after inoculation monophialides with in situ microconidia were observed over the surface of the leaves. Based on the evidence and previous studies, internal hyphae colonization of F. solani f. sp. passiflorae concentrates on the collar area and the damages of the cells indicate an extracellular enzymatic activity of the fungus. The incubation and latent periods of F. solani f. sp. passiflorae were 1.4 and 4 days, respectively. © 2017. Acad. Colomb. Cienc. Ex. Fis. Nat.References
Agrios, G. N. (2005). Plant pathology (Vol. 5). Burlington: Elsevier Academic Press.
Bateman, D. F., & Basham, H. G. (1976). Degradation of plant cell walls and membranes by microbial enzymes. In R. Heitefuss, & P.H. Williams, (eds.). Physiological Plant Pathology. (pp.316-355). Berlin: Springer Berlin Heidelberg.
Bishop, C. D., & Cooper, R. M. (1983). An ultrastructural study of vascular colonization in three vascular wilt diseases I. Colonization of susceptible cultivars. Physiological Plant Pathology, 23 (3): 323-343.
Bueno, C. J., Fischer, I. H., Rosa, D. D, & Furtado, E. L. (2009). Production of extracellular enzymes by Fusarium solani from yellow passionfruit. Tropical Plant Pathology, 34 (5):343-346.
Bueno, C. J., Fischer, I. H., Rosa, D. D., Firmino, A. C., Harakava, R., Oliveira, C. M. G., & Furtado, E. L. (2014). Fusarium solani f. sp. passiflorae: a new forma specialis causing collar rot in yellow passion fruit. Plant Pathology, 63 (2): 382-389.
Castaño-Zapata, J. (2009). Important diseases of Passifloraceae in Colombia. In D. Miranda, G. Fischer, C. Carranza, S. Magnitskiy, F. Casierra, W. Piedrahíta, & L.E. Flórez (eds.). Cultivo, Poscosecha y Comercialización de las Pasifloráceas en Colombia: Maracuyá, Granadilla, Gulupa y Curuba. (pp.223-245). Bogotá: Sociedad Colombiana de Ciencias Hortícolas.
Cavichioli, J. C., Corrêa, L. de S., Boliani, A. C., & Dos Santos, C. P. (2011). Growth and yield of yellow passionfruit grafted on three roostocks. Revista Brasileira de Fruticultura, 33 (2): 558-566.
Fischer, I. H., de Almeida, A. P., Fileti, M. de S., Bertani, R. M. de A., de Arruda, M. C., & Bueno, C. J. (2010). Evaluation of passifloraceas, fungicides and Trichoderma for passionfruit collar rot handling, caused by Nectria haematococca. Revista Brasileira de Fruticultura, 32 (3):709-717.
Fischer, I. H., Lourenço, S. A., Martins, M. C., Kimati, H., & Amorim, L. (2005). Selection of resistant plants and fungicides for the control of passionfruit collar rot, caused by Nectria haematococca. Fitopatologia Brasileira, 30 (3):250-258.
Flores, P. S., Otoni, W. C., Dhingra, O. D., de Souza Diniz, S. P. S., dos Santos, T. M., & Bruckner, C. H. (2012). In vitro selection of yellow passionfruit genotypes for resistance to Fusarium vascular wilt. Plant Cell, Tissue and Organ Culture, 108 (1): 37-45.
Gao, H., Chen, J., He, J., Ning, J., Yu, B., Liu, J., & Ha, J. (2004). The character of cell wall degradation enzyme produced by Fusarium graminearum. Journal of Maize Sciences, 13 (3):112-113.
Gardner, D. E. (1989). Pathogenicity of Fusarium oxysporum f. sp. passiflorae to banana poka and other Passiflora spp. in Hawaii. Plant Disease, 73 (6): 476-478.
Isaacs, M. (2009). National and international markets of Passifloraceae fruit crops. In D. Miranda, G. Fischer, C. Carranza, S. Magnitskiy, F. Casierra, W. Piedrahíta, & L.E. Flórez (eds.). Cultivo, Poscosecha y Comercialización de las Pasifloráceas en Colombia: Maracuyá, Granadilla, Gulupa y Curuba. (pp.327-344). Bogotá: Sociedad Colombiana de Ciencias Hortícolas.
Jackowiak, H., Packa, D., Wiwart, M., & Perkowski, J. (2005). Scanning electron microscopy of Fusarium damaged kernels of spring wheat. International Journal of Food Microbiology, 98 (2): 113-123.
Kang, Z., & Buchenauer, H. (2002). Studies on the infection process of Fusarium culmorum in wheat spikes: degradation of host cell wall components and localization of trichothecene toxins in infected tissue. European Journal of Plant Pathology, 108 (7): 653-660.
Köller, W., Allan, C. R., & Kolattukudy, P. E. (1982). Role of cutinase and cell wall degrading enzymes in infection of Pisum sativum by Fusarium solani f. sp. pisi. Physiological Plant Pathology, 20 (1): 47-60.
Leslie, J., & Summerell, B. (2006). The Fusarium laboratory manual. New York: Blackwell Publishing Professional. Londoño, J. (2012). Evaluación de la resistencia genética de especies de Passiflora spp. a Fusarium spp. agente causal de la secadera. (M.Sc. Thesis), Universidad Nacional de Colombia, Palmira.
Meiting, L., & Shaosheng, Z. (2010). Induction of extracellular cell wall-degrading enzymes from Fusarium oxysporum f. sp. cubense and their effect on degradation of banana tissue. Chinese Agricultural Science Bulletin, 5: 048.
Meyer, D., Weipert, D., & Mielke, H. (1986). Effects of Fusarium culmorum infection on wheat quality. Getreide Mehl. Brot., 40:35-39.
Miranda, P. M., Carvalho, C. R., Marcelino, F. C., Andréia, M., & Mendonça, C. (2008). Morphological aspects of Passiflora edulis f. flavicarpa chromosomes using acridine orange banding and rDNA-FISH tools. Caryologia, 61 (2): 154-159.
Montanher, A. B., Zucolotto, S. M., Schenkel, E. P, & Fröde, T. S. (2007). Evidence of anti-inflammatory effects of Passiflora edulis in an inflammation model. Journal of Ethnopharmacology, 109 (2): 281-288.
Mullen, J. M., & Bateman, D. F. (1975). Enzymatic degradation of potato cell walls in potato virus X-free and potato virus X-infected potato tubers by Fusarium roseum ‘Avenaceum’. Phytopathology, 65 (7): 797-802.
Nelson, P. E., Dignani, M. C., & Anaissie, E. J. (1994). Taxonomy, biology, and clinical aspects of Fusarium species. Clinical Microbiology Reviews, 7 (4): 479-504.
Nelson, P. E., Toussoun, T. A., & Marasas, W. F. O. (1983). Fusarium species: an illustrated manual for identification. Pennsylvania: Pennsylvania State University Press. Ocampo, J. (2007). Study of the genetic diversity of genus Passiflora L. (Passifloraceae) and its distribution in Colombia. (Ph.D. Thesis) Montpellier: Ecole Nationale Supérieure d ́Agronomie de Montpellier – SupAgro.
Ocampo, J., Urrea, R., Wyckhuys, K., & Salazar, M. (2013). Exploration of the genetic variability of yellow passionfruit (Passiflora edulis f. flavicarpa Degener) as basis for a breeding program in Colombia. Acta Agronómica, 62 (4): 352-360.
Oliveira-Freitas, J. C. de, Viana, A. P., Santos, E. A., Paiva, C. L., de Lima e Silva, F. H., do Amaral Jr., A. T., & Dias, V. M. (2016). Resistance to Fusarium solani and characterization of hybrids from the cross between P. mucronata and P. edulis. Euphytica, 208 (3): 493-507.
Ortíz, E., & Hoyos, L. (2012). Description of the symptomatology associated with fusariosis and its comparison with other diseases for the purple passionfruit (Passiflora edulis Sims.) in the Sumapaz region (Colombia). Revista Colombiana de Ciencias Hortícolas, 6 (1): 110-116.
Ortíz, E., Cruz, M., Melgarejo, L. M., Marquínez, X., & Hoyos- arvajal, L. (2014). Histopathological features of infections caused by Fusarium oxysporum and F. solani in purple passionfruit plants (Passiflora edulis Sims). Summa Phytopathologica, 40 (2): 134-140.
Patel, S. S. (2009). Morphology and pharmacology of Passiflora edulis: a review. Journal of Herbal Medicine and Toxicology, 3 (1): 1-6.
Pietro, A. D., Madrid, M. P., Caracuel, Z., Delgado-Jarana, J., & Roncero, M. I. G. (2003). Fusarium oxysporum: exploring the molecular arsenal of a vascular wilt fungus. Molecular Plant Pathology, 4 (5): 315-325.
Preisigke, S. da C., Martini, F. V., Rossi, A. A. B., Serafim, M. E., Barelli, M. A. A., da Luz, P. B., & Neves, L. G. (2015a). Genetic variability of Passiflora spp. against collar rot disease. Australian Journal of Crop Science, 9 (1): 69-74.
Preisigke, S. da C., Neves, L. G., Araújo, K. L., Barbosa, N. R., Serafim, M. E., & Krause, W. (2015b). Multivariate analysis for the detection of Passiflora species resistant to collar rot. Bioscience Journal, 31 (6): 1700-1707.
Rogers, L. M., Flaishman, M. A., & Kolattukudy, P. E. (1994). Cutinase gene disruption in Fusarium solani f. sp. pisi decreases its virulence on pea. The Plant Cell, 6 (7): 935-945.
Saniewska, A., Dyki, B., & Jarecka, A. (2004). Morphological and histological changes in tulip bulbs during infection by Fusarium oxysporum f. sp. tulipae. Phytopathologia Polonica, 34: 21-39.
Schneider, C. A., Rasband, W. S., & Eliceiri, K. W. (2012). NIH Image to ImageJ: 25 years of image analysis. Nature Methods, 9 (7): 671-675.
Schwarz, P., Casper, H., Barr, J., & Musial, M. (1997). Impact of Fusarium head blight on the malting and brewing quality of barley. Cereal Research Communications, 813-814.
Shaykh, M., Soliday, C., & Kolattukudy, P. E. (1977). Proof for the production of cutinase by Fusarium solani f. pisi during penetration into its host, Pisum sativum. Plant Physiology, 60 (1): 170-172.
Short, G.E., & Lacy, M.L. (1974). Germination of Fusarium solani f. sp. pisi chlamydospores in the spermosphere of pea. Phytopathology, 64: 558-562.
Silva, S., Oliveira, E. J. de, Haddad, F., Laranjeira, F. F., Jesus, N. de, Oliveira, S. A. S. de, … do Amaral Jr., A. T. (2013). Identification of passionfruit genotypes resistant to Fusarium oxysporum f. sp. passiflorae. Tropical Plant Pathology, 38 (36): 236-242.
Souza, M. M. de, Santana-Pereira, T. N., Hoffmann, M., Melo, E. J. T. de, & Pereira-Louro, R. (2002). Embryo sac development in yellow passionfruit Passiflora edulis f. flavicarpa (Passifloraceae). Genetics and Molecular Biology, 24 (4): 471-475.
Vaca-Vaca, J. C., Carrasco-Lozano, E. C., Rodríguez-Rodríguez, M., Betancur-Perez, J. F. and López-López, K. (2016). First report of a begomovirus presents in yellow passionfruit [Passiflora edulis f. flavicarpa (Degener)] in Valle del Cauca, Colombia. Revista Colombiana de Biotecnología, 18 (2): 56-65.
Van der Plank, J. E. (1963). Plant diseases: epidemics and control. New York: Elsevier Academic Press.
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