Abstract
In this study the antioxidant and antitrosative capacity of the extracts and isolated flavonoids from the leaves and bark of Bauhinia kalbreyeri Harms (Cow Hoof. Fabaceae) was examinated. The extracts showed high antioxidant and antinitrosative functionality, while the flavonoids ability to capture metals and inhibit the NO. Significative differences were found among the extracts, and into those and the flavonoids fractions (p < 0.05). The antioxidant activity of the plant seems to be based in the whole phenolic derivatives. The results obtained indicate that the antioxidant potential of B. kalbreyeri is comparable with the Butylated Hydroxytoluene and the ascorbic acid used as antioxidants by the food and pharmaceutical industry.
Keywords
References
Ali, M. S., Azhar, i. & Amtul, Z. 1999. Antimicrobial screening of Caesalpiniaceae. Fitoterapia 70: 299-304.
Bencheroun, M. N., Pourquier, P., Schott., B. & Robert, J. 1993. Dexorubicin-induced lipid peroxidation and glutatione peroxidase activity in tumor cell lines selected for resistance to dexorubicin. Eur. J. Biochem. 211: 141-146.
Da Silva, M. L & Filho, V. C. 2002. Plantas do gênero Bauhinia. Composição química e potencial farmacológico. Química nova. 25(3): 449-454.
Damasceno, D. C., Volpato, G. T., Mattos, I., Calderon, P., Aguilar, R. & Cunha Rudge, M. V. 2004. Effect of Bauhinia forficata extracts in diabetic pregnant rats: maternal repercussions. Phytomedicine. 11: 196-201.
Dasgupta, N. & De, B. 2007. Antioxidants Activity of some leafy vegetables of India: A comparative study. Food Chemistry. 101: 471-474.
Dinis, T. C. P., Madeira, V. M. C. & Almeida, L. M. 1994. Action of phenolic derivatives (acetoaminophen, salycilate and 5-aminosalycilate) as inhibitors of membrane lipid peroxidation and as perodyl radical scavengers. Archives of biochemistry and biophysics. 315: 161-169.
Duarte-Almeida, J. M., Negri, G. & Salatin, A. 2004. Volatile oils in leaves of Bauhinia (Fabaceae Caesalpinioideae). Biochemical Systematics and Ecology. 32(8): 747-753.
Dumoulin, M. J., Chaine, R., Atanasiv, R., Nadeau, R. & Mateescu, M. 1996. Comparative antioxidant and cardioprotective effects of ceruloplasmin, superoxide dismutase and albumin. Arzneimittel-fors-chung-drug-research. 46: 588-861.
Giraldo, B., Hernández, M. M., Angulo, P. & Fuertes, C. 2003. Actividad antinitrosativa y antiinflamatoria de los flavonoides de las hojas de Uncaria tomentosa Willd. D.C. (Uña de gato). Rev. Soc. Quím. Perú. 69(4): 229-242.
Goeth, M. E., Freyberger, A. & Rieder, P. 1990. Oxidate stress: A role in the pathogenesis of parkinson’s disease. J Neural Transm. 29: 241-249.
Gorinstein, S., Cvikrová, M., Machackova, I., Haruenkit, R., Park, Y.-S., Jung, S.-T.; Yamamoto, K., Martinez, A. L., Katrich, E. & Trakhtenberg, S. 2004. Food Chemistry. 84: 503-510.
Gülçin, I., Büyükokuroðlu, M. E., OKTAY, M. & Küfrevioðlu, Ö. I.. 2003. Antioxidant and analgesic activities of turpentine of Pinus nigra Arn. Subs. Pallsiana (Lamb.) Holmboe. Journal of Ethnopharmacology. 86: 51-58.
Gupta, M., Mazumder, U. K., Sambath Kumar, Gomathi, P., Rajeshwar, Y., Kakoti, B. B., Tamil Selven, V. 2005. Antiinflammatory, analgesic and antipyretic effects of methanol extract from B. racemosa stem bark in animal models. Journal of Ethnopharmacology. 98(3): 267-273.
Grutteridge, J. M. 1985. Inhibition of the fenton reaction by the protein caeruloplasmin and other copper complexes. Assess ment of ferroxidase and radical scavenging activities. Chenico-Bilogical Interactions. 56: 113-120.
Halliwell, B., Gutteridge, J. M. C., & Cross, C. E. 1992. Free radicals, antioxidants and human disease: Where are we now? J. Lab. Clin. Med. 119:598-620.
Kumaran, A. & Karunakaran, R. J. 2007. In vitro antioxidant activities of methanol extracts of .ve Phyllanthus species from India. Swiss Society of Food Science and Technology. Published by Elsevier. p. v.
Lindahl, M., & Tagesson C. 1997. Flavonoids as phospholipase A2 inhibitors: importance of their structure for selective inhibition of group II phospholipase A2. Inflammation Vol. 21: 347-56.
Matos, F. J. A. 1998. Farmácias vivas: sistema de utilizacão de plantas medicinais projetado para pequenas comunidades. 2°ed: Fotaleza: EUFC. Brazil.
Mathew, S. & Abraham, E. 2006. In vitro antioxidant activity and scavenging efects of Cinnamomum verum leaf extract assayed by diferent methodologies. Food and Chemical Toxicology. 44: 198-206.
Murillo, E., Tique, M .M., Ospina, L F. & Lombo Ó. 2006. Evaluación preliminar de la actividad hipoglicemiante en ratones diabéticos por aloxano y capacidad antioxidante in vitro de extractos de Bauhinia kalbreyeri Harms. Rev. Col. Cienc. Quím. Farm. 35(1): 64-80.
Ohinishi, M. 2005. Inhibitory effects of chlorogenic acids on linoleic acid peroxidation and haemolysis, citado por Miceli, N. et al. Anti-inflammatory activity of extract and fractions from Nepeta sibthorpii Bentham.. Journal of Ethnopharmacology. 97(2):261-266.
Oyaizu, M. 1986. Studies on product of browning reaction prepared from glucose amine. Japanese journal of nutrition. 44: 307-315.
Pietta, P-G. 2000. Flavonoids as antioxidants. Reviews; J.Nat. Prod. 63: 1035-1042.
Rajkapoor, B., Jayakar, B. & Murugesh, N. 2003. Antitumour activity of Bauhinia variegata on Dalton’s ascitic lymphoma. Journal of Ethnopharmacology. 89: 107-109.
Reddy, M. V. B., Reddy, M. K., Gunasekar, D., Caux, C. & Bodo, B. 2003. A flavanone and dihydrodibenzoxepin from Bauhinia variegata. Phytochemistry 64: 879-882.
Rice-Evans, C. A., Miller, N.J., & Pagang, G. 1996. Structure-antioxidant activity relationships of flavonoids and phenolic acids. Free radical & medicine. 20(7): 933-956.
Ruch, R. J., Cheng, S. J. & Klauning, J. E. 1989. Prevention of cytotoxixity and inhibition of intracellular communication by antioxidant catechins isolated from Chinese green tea. Carcinogenesis. 10: 1003-1008.
Russo, E. M. K., Reichelt, A. A. J., De-Sa, J. R., Furlanetto, R. P., Moises R. C. S., Kasamatsu, T. S. & Chacra A. R. 1990.
Clinical trial of Myrcia uniflora and Bauhinia forficata leaf extracts in normal and diabetic patients. Brazilian Journal of Medical and Biological Research. 23: 11-20.
Saha, K., Lajis N. H., Israf D. A., Hamzah A. S., Khozirah, S., Khamis, S., & Syahida , A . 2004. Evaluation of antioxidant and nitric oxide inhibitor y activities of selected Malaysi an medicinal plants. Journal of Ethnopharmacology. 92: 263-267. Sanchez-Moreno, C., Larrauri, J. A. & Saura-Calixto, F. 1999.
Free radical scavenging capacity an inhibition of lipid oxidation of wines, grape juices and related polyphenolic constituents. Food Research International. 32: 407-412.
Shahidi, F., & Wanasundara, P. K .J. P. D. 1992. Phenolic antioxidants. Critica Reviews in Food Science and Nutrition. 32: 67-103.
Silva, K. L. Monografia de Conclusão de Curso, Universidade do Vale do Itajaí, Brasil, 1999.
Singleton & Rossi, J.A. 2006. Colorimetry of total phenols with phospho molybdic phoshphotungstic acid reagents, citado por Mathew, Sindhu & Abraham, E. In vitro antioxidant activity and scavenging effects of Cinnamomum verum leaf extract assayed by diferent methodologies. Food and Chemical Toxicology. 44:198–206.
Soares, J., Costa, S. & Cecim, M. 2000. Níveis glicêmicos de colesterol em ratos com diabetes melittus aloxano induzido, tratados com infusão de Bauhinia candicans ou Syzygium jambolanum. Ciência Rural, Brasil. 30(1): 113-118.
Soobrattee, N. A., Neergheen, V. S., Luximan-Ramma, A. O., Auroma, C. & Bahorum, T. 2005. Phenolics as potencial antioxidants therapeutic agents: Mechanism and actions. Mutation Research. 579: 200-213.
Sudheesh, S., Sandhya, C., Sarah, K. A., & Vijayalakshmi, N. R. 1999. Antioxidant activity of flavonoids from Solanum melongena. Phytother Res. 13: 393-396.
Viana, E. P., Santa-Rosa, R. S., Almeida, S. S. M. S., y Santos, L. S. Constituents of the stem bark of B. guianensis. Fitoterapia. 70:111-112.
Wazlawilk, E., Silva, M. A., Peters, R. R., Simões, C. M. O. & Ribeiro-Do-Vale, R. M. 1994. IX Reunião Anual da FSBE, Caxambú, Brasil.
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