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- Elena E. Stashenko, Jairo René Martínez, Diego Camilo Durán, Yuri Córdoba, Deyanira Caballero, Comparative study of chemical composition and antioxidant activity of essential oils of some plants from genus Lippia (Verbenaceae) grown in Colombia , Revista de la Academia Colombiana de Ciencias Exactas, Físicas y Naturales: Vol. 38 (2014): Suplemento Vol. 38
- Edwin Alexander Rodríguez, Anderson Julián Árias, Eva Giselle Vásquez, Jairo René Martínez, Elena E. Stashenko, YIELD AND ANTIOXIDANT CAPACITY OF ROSMARINUS OFFICINALIS, SALVIA OFFICINALIS AND PSIDIUM GUAJAVA EXTRACTS OBTAINED WITH SUPERCRITICAL CO2 , Revista de la Academia Colombiana de Ciencias Exactas, Físicas y Naturales: Vol. 36 No. 140 (2012)
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Abstract
This work reports on the study of secondary metabolites obtained by means of different extraction techniques from three Verbenaceae plants, grown in Colombia’s northeast. Lippia alba was a new chemotype not previously described in the literature. It contains carvone (40-57%) as the main component of the oils, obtained by hydrodistillation (HD) and microwave-assisted hydrodistillation (MWHD), and of the extracts isolated by simultaneous distillation-extraction (SDE), and supercritical fluid extraction (SFE). Other main components were limonene (24-37%), bicyclosesquiphellandrene (2-22%), piperitenone (1-2%), piperitone (0.8-1.2%) and ß–bourbonene (0.6-1.5%). Volatile fractions obtained by static (SHS), dynamic (purge and trap, P & T) headspace methods, or headspace-solid phase microextraction (HS-SPME), contained limonene (27-77%) as the most abundant component, followed by carvone (14-30%), bicyclosesquiphellandrene (1-33%), and ß–bourbonene (0.5-6.5%). The main components found in the essential oils (HD, MW) and extracts (SDE, SFE) of Aloysia triphylla, were neral (19-22%) and geranial (33-38%), followed by nerol (2-5%) and geraniol (2-6%), the sesquiterpenoids ar-curcumene (3-4%), bicyclosesquiphellandrene (2-3%), spathulenol (2-4%), nerolidol (ca. 2%), ß-bourbonene (1-2%), trans-ß-caryophyllene (3-5%) and its oxide (1-1.5%). The Lantana camara essential oil was of the sesquiterpenoid type; over 60% of its composition was represented by sesquiterpene hydrocarbons, C15H24, and ca. 30% by their oxygenated derivatives. trans-ß-Caryophyllene (14-15%) and its oxide (4- 9%), bicyclosesquiphellandrene (16-18%), α-zingiberene (5-6%), E,E-α-farnesene (3-4%) and γbisabolene (3-4%) were the main sesquiterpenes isolated from L. camara. The monoterpene fraction, C10H16, in L. camara did not reach 10%; ß-pinene (0.2-3%) and ß-myrcene (2-4%) were the main monoterpene hydrocarbons. While in L. alba monoterpenes (>30%) and oxygenated monoterpenes (ca. 55%) were dominant, in A. triphylla, oxygenated monoterpenes (>70%) prevailed, ca. 60% was represented by citral. On the other hand, L. camara was poor in monoterpenes and their oxygenated derivatives; most of its secondary metabolites were sesquiterpene hydrocarbons (ca. 60%) and their oxygenated derivatives (ca. 30%). The essential oils of the three Verbenaceae plants studied exhibited an in vitro antioxidant effect, which was similar or higher than those of vitamin E or BHA, when measured in a model lipid system (linoleic acid emulsio) at the same concentration, e.g. 10 g/L. L. camara oil was the most active, followed by L. alba oil.
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References
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